Product Pathways - Cytoskeletal Signaling
EpCAM (VU1D9) Mouse mAb (Alexa Fluor® 647 Conjugate) #5447
PhosphoSitePlus® protein, site, and accession data: EPCAM
| Applications | Reactivity | Sensitivity | Isotype |
|---|---|---|---|
| IF-IC F | H | Endogenous | Mouse IgG1 |
Applications Key:
IF-IC=Immunofluorescence (Immunocytochemistry)
F=Flow Cytometry
Reactivity Key:
H=Human
Species cross-reactivity is determined by western blot. Species enclosed in parentheses are predicted to react based on 100% sequence homology.
Protocols
- 5447:
- Flow, Immunofluorescence
Specificity / Sensitivity
EpCAM (VU1D9) Mouse mAb (Alexa Fluor® 647 Conjugate) detects endogenous levels of total EpCAM protein.
Source / Purification
Monoclonal antibody is produced by immunizing animals with NCI-H69 small cell lung carcinoma cells.
Flow Cytometry
Flow cytometric analysis of unpermeabilized HT-29 cells using EpCAM (VU1D9) Mouse mAb (Alexa Fluor® 647 Conjugate) (green) compared to Mouse (MOPC-21) mAb IgG1 Isotype Control (Alexa Fluor® 647 Conjugate) #4843 (red).
IF-IC
Confocal immunofluorescent analysis of HT-29 cells using EpCAM (VU1D9) Mouse mAb (Alexa Fluor® 647 Conjugate) (blue pseudocolor) and Di-Methyl-Histone H3 (Lys4) (C64G9) Rabbit mAb #9725 (green).
Description
This Cell Signaling Technology antibody is conjugated to Alexa Fluor® 647 fluorescent dye and tested in-house for direct flow cytometry and immunofluorescent analysis in human cells. The antibody is expected to exhibit the same species cross-reactivity as the unconjugated EpCAM (VU1D9) Mouse mAb #2929.
Background
Epithelial cell adhesion and activating molecule (EpCAM/CD326) is a transmembrane glycoprotein that mediates Ca2+-independent, homophilic adhesions on the basolateral surface of most epithelial cells. EpCAM is not expressed in adult squamous epithelium, but it is highly expressed in adeno and squamous cell carcinomas (1). Research studies identified EpCAM as one of the first tumor-associated antigens, and it has long been a marker of epithelial and tumor tissue. Investigators have shown that EpCAM is highly expressed in cancer cells (reviewed in 2,3).
- Went, P.T. et al. (2004) Hum Pathol 35, 122-8.
- Baeuerle, P.A. and Gires, O. (2007) Br J Cancer 96, 417-23.
- Armstrong, A. and Eck, S.L. (2003) Cancer Biol Ther 2, 320-6.
Application References
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Companion Products
- 5198 EpCAM (VU1D9) Mouse mAb (Alexa Fluor® 488 Conjugate)
- 2929 EpCAM (VU1D9) Mouse mAb
- 4843 Mouse (MOPC-21) mAb IgG1 Isotype Control (Alexa Fluor® 647 Conjugate)
For Research Use Only. Not For Use In Diagnostic Procedures.
