Product Pathways - Lineage Markers
Nanog (D73G4) XP® Rabbit mAb (Alexa Fluor® 647 Conjugate) #5448
|IF-IC F||H (Mk)||Endogenous||Rabbit IgG|
Reactivity Key: H=Human Mk=Monkey
Species cross-reactivity is determined by western blot. Species enclosed in parentheses are predicted to react based on 100% sequence homology.
Specificity / Sensitivity
Nanog (D73G4) XP® Rabbit mAb (Alexa Fluor® 647 Conjugate) detects endogenous levels of total nanog protein.
Source / Purification
Monoclonal antibody is produced by immunizing animals with a synthetic peptide corresponding to residues near the amino terminus of human nanog protein.
Flow cytometric analysis of HeLa cells (blue) and NTERA-2 cells (green) using Nanog (D73G4) XP® Rabbit mAb (Alexa Fluor® 647 Conjugate).
This Cell Signaling Technology antibody is conjugated to Alexa Fluor® 647 fluorescent dye and tested in-house for direct flow cytometry and immunofluorescent analysis in human cells. The antibody is expected to exhibit the same species cross-reactivity as the unconjugated Nanog (D73G4) XP® Rabbit mAb #4903.
Nanog is a homeodomain-containing transcription factor that is essential for the maintenance of pluripotency and self renewal in embryonic stem cells (1). Nanog expression is controlled by a network of factors including Sox2 and the key pluripotency regulator Oct-4 (1). Recent advances in somatic cell reprogramming have utilized viral expression of combinations of transcription factors including nanog, Oct-4, Sox2, KLF4, c-Myc, and LIN28 (2,3).
- Kim, J. et al. (2008) Cell 132, 1049-61.
- Takahashi, K. et al. (2007) Nat Protoc 2, 3081-9.
- Yu, J. et al. (2007) Science 318, 1917-20.
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For Research Use Only. Not For Use In Diagnostic Procedures.