Cell Signaling Technology
XP Monoclonal Antibody

Product Pathways - Lineage Markers

Nanog (D73G4) XP® Rabbit mAb (Alexa Fluor® 647 Conjugate) #5448

Applications Reactivity Sensitivity Isotype
IF-IC F H (Mk) Endogenous Rabbit IgG

Applications Key:  IF-IC=Immunofluorescence (Immunocytochemistry)  F=Flow Cytometry
Reactivity Key:  H=Human  Mk=Monkey
Species cross-reactivity is determined by western blot. Species enclosed in parentheses are predicted to react based on 100% sequence homology.

Protocols

Specificity / Sensitivity

Nanog (D73G4) XP® Rabbit mAb (Alexa Fluor® 647 Conjugate) detects endogenous levels of total nanog protein.

Source / Purification

Monoclonal antibody is produced by immunizing animals with a synthetic peptide corresponding to residues near the amino terminus of human nanog protein.

Flow Cytometry

Flow Cytometry

Flow cytometric analysis of HeLa cells (blue) and NTERA-2 cells (green) using Nanog (D73G4) XP® Rabbit mAb (Alexa Fluor® 647 Conjugate).

IF-IC

IF-IC

Confocal immunofluorescent analysis of NTERA-2 cells (left) and HeLa cells (right) using Nanog (D73G4) XP® Rabbit mAb (Alexa Fluor® 647 Conjugate) (blue pseudocolor). Actin filaments were labeled with DY-554 phalloidin (red).

Description

This Cell Signaling Technology antibody is conjugated to Alexa Fluor® 647 fluorescent dye and tested in-house for direct flow cytometry and immunofluorescent analysis in human cells. The antibody is expected to exhibit the same species cross-reactivity as the unconjugated Nanog (D73G4) XP® Rabbit mAb #4903.

Background

Nanog is a homeodomain-containing transcription factor that is essential for the maintenance of pluripotency and self renewal in embryonic stem cells (1). Nanog expression is controlled by a network of factors including Sox2 and the key pluripotency regulator Oct-4 (1). Recent advances in somatic cell reprogramming have utilized viral expression of combinations of transcription factors including nanog, Oct-4, Sox2, KLF4, c-Myc, and LIN28 (2,3).

  1. Kim, J. et al. (2008) Cell 132, 1049-61.
  2. Takahashi, K. et al. (2007) Nat Protoc 2, 3081-9.
  3. Yu, J. et al. (2007) Science 318, 1917-20.

Application References

Have you published research involving the use of our products? If so we'd love to hear about it. Please let us know!

Companion Products

For Research Use Only. Not For Use In Diagnostic Procedures.

Products