Cell Signaling Technology

Product Pathways - Neuroscience

BRSK2 (D29B6) Rabbit mAb #5460

Applications Reactivity Sensitivity MW (kDa) Isotype
W IP H M R Endogenous 78-90 Rabbit IgG

Applications Key:  W=Western Blotting  IP=Immunoprecipitation
Reactivity Key:  H=Human  M=Mouse  R=Rat
Species cross-reactivity is determined by western blot. Species enclosed in parentheses are predicted to react based on 100% sequence homology.

Protocols

Specificity / Sensitivity

BRSK2 (D29B6) Rabbit mAb detects endogenous levels of total BRSK2 protein.

Source / Purification

Monoclonal antibody is produced by immunizing animals with a synthetic peptide corresponding to residues near the amino terminus of human BRSK2 protein.

Western Blotting

Western Blotting

Western blot analysis of extracts from mouse and rat brain using BRSK2 (D29B6) Rabbit mAb.

Background

Brain-specific kinase 1 (BRSK1; SAD-B) and Brain-specific kinase 2 (BRSK2; SAD-A) are serine/threonine kinases closely related to AMPK. LKB1 phosphorylates Thr189 in the T-loop of BRSK1 and Thr174 in the T-loop of BRSK2, resulting in activation of the kinases (1). BRSK1 localizes to synaptic vesicles in the hippocampus and cerebellum, together with the active zone proteins Bassoon and CAST, and BRSK1 phoshorylates the active zone protein RIM1 (2). An alternatively spliced from of BRSK1 displays unique activity during the cell cycle, phosphorylating Ser131 of γ-tubulin and controling centrosome duplication (3). Neuronal polarization, including axon formation, is fundamental for normal brain development. BRSK1 -/- and BRSK2 -/- mice have defects in neuronal polarity and impaired corticogenesis (4). Knockdown of BRSK1 and BRSK2 in vitro diminishes axonal growth (5).

  1. Bright, N.J. et al. (2008) J Biol Chem 283, 14946-54.
  2. Inoue, E. et al. (2006) Neuron 50, 261-75.
  3. Alvarado-Kristensson, M. et al. (2009) Nat Cell Biol 11, 1081-92.
  4. Kishi, M. et al. (2005) Science 307, 929-32.
  5. Choi, Y.J. et al. (2008) Genes Dev 22, 2485-95.

Application References

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Companion Products

For Research Use Only. Not For Use In Diagnostic Procedures.

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