Product Pathways - Neuroscience
Synaptophysin (D35E4) XP® Rabbit mAb #5461
PhosphoSitePlus® protein, site, and accession data: SYP
| Applications | Reactivity | Sensitivity | MW (kDa) | Isotype |
|---|---|---|---|---|
| W IP IF-F | H M R | Endogenous | 38 | Rabbit IgG |
Applications Key:
W=Western Blotting
IP=Immunoprecipitation
IF-F=Immunofluorescence (Frozen)
Reactivity Key:
H=Human
M=Mouse
R=Rat
Species cross-reactivity is determined by western blot. Species enclosed in parentheses are predicted to react based on 100% sequence homology.
Protocols
Specificity / Sensitivity
Synaptophysin (D35E4) XP® Rabbit mAb detects endogenous levels of total synaptophysin protein. Some nuclear background staining was observed by confocal immunofluorescent analysis.
Source / Purification
Monoclonal antibody is produced by immunizing animals with a synthetic peptide surrounding Ala230 of human synaptophysin protein.
Western Blotting
Western blot analysis of extracts from mouse and rat brain using Synaptophysin (D35E4) XP® Rabbit mAb.
IF-F
Confocal immunofluorescent image of mouse brain labeled with Synaptophysin (D35E4) XP® Rabbit mAb (green) and β3-Tubulin (TU-20) Mouse mAb #4466 (red). Blue pseudocolor = DRAQ5® #4084 (fluorescent DNA dye).
Background
Synaptophysin (SYP) is a neuronal synaptic vesicle glycoprotein that is expressed in neuroendocrine cells and neoplasms (1). Synaptophysin contains four transmembrane domains that form a hexameric channel or gap junction-like pore (2). Synaptophysin binds to the SNARE protein synaptobrevin/VAMP, which prevents the inclusion of synaptobrevin in the synaptic vesicle fusion complex and creates a pool of synaptobrevin for exocytosis when synapse activity increases (3). Synaptophysin is also responsible for targeting synaptobrevin 2/VAMP2 to synaptic vesicles, a critical component of the fusion complex (4).
- Wiedenmann, B. et al. (1986) Proc Natl Acad Sci USA 83, 3500-4.
- Valtorta, F. et al. (2004) Bioessays 26, 445-53.
- Arthur, C.P. and Stowell, M.H. (2007) Structure 15, 707-14.
- Bonanomi, D. et al. (2007) Biochem J 404, 525-34.
Application References
Have you published research involving the use of our products? If so we'd love to hear about it. Please let us know!
Companion Products
- 3347 SYT1 Antibody
- 3926 SNAP25 (D110) Antibody
- 2869 Syntaxin 6 (C34B2) Rabbit mAb
- 3757 SNIP/p140Cap Antibody
- 2145 NSF Antibody
- 7071 Phototope®-HRP Western Blot Detection System, Anti-rabbit IgG, HRP-linked Antibody
- 7074 Anti-rabbit IgG, HRP-linked Antibody
- 7075 Anti-biotin, HRP-linked Antibody
- 7727 Biotinylated Protein Ladder Detection Pack
- 7003 20X LumiGLO® Reagent and 20X Peroxide
- 9997 Tris Buffered Saline with Tween 20 (TBST-10X)
- 9998 BSA
- 9999 Nonfat Dry Milk
For Research Use Only. Not For Use In Diagnostic Procedures.
