Product Pathways - Neuroscience
Synaptophysin (D35E4) XP® Rabbit mAb #5461
|5461S||100 µl (10 western blots)||---||In Stock||---|
|5461P||40 µl (4 western blots)||---||In Stock||---|
|5461||carrier free and custom formulation / quantity||email request|
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|W||1:1000||Human, Mouse, Rat||Endogenous||38||Rabbit IgG|
Species cross-reactivity is determined by western blot.
Applications Key: W=Western Blotting, IP=Immunoprecipitation, IF-F=Immunofluorescence (Frozen)
Specificity / Sensitivity
Synaptophysin (D35E4) XP® Rabbit mAb detects endogenous levels of total synaptophysin protein. Some nuclear background staining was observed by confocal immunofluorescent analysis.
Source / Purification
Monoclonal antibody is produced by immunizing animals with a synthetic peptide surrounding Ala230 of human synaptophysin protein.
Western blot analysis of extracts from mouse and rat brain using Synaptophysin (D35E4) XP® Rabbit mAb.
Synaptophysin (SYP) is a neuronal synaptic vesicle glycoprotein that is expressed in neuroendocrine cells and neoplasms (1). Synaptophysin contains four transmembrane domains that form a hexameric channel or gap junction-like pore (2). Synaptophysin binds to the SNARE protein synaptobrevin/VAMP, which prevents the inclusion of synaptobrevin in the synaptic vesicle fusion complex and creates a pool of synaptobrevin for exocytosis when synapse activity increases (3). Synaptophysin is also responsible for targeting synaptobrevin 2/VAMP2 to synaptic vesicles, a critical component of the fusion complex (4).
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For Research Use Only. Not For Use In Diagnostic Procedures.
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Cell Signaling Technology® is a trademark of Cell Signaling Technology, Inc.