Product Pathways - Motif Antibodies
Phospho-Tyrosine Mouse mAb (P-Tyr-100) (HRP Conjugate) #5465
| Applications | Reactivity | Sensitivity | Isotype |
|---|---|---|---|
| W E-P | Endogenous | Mouse IgG1 |
Applications Key:
W=Western Blotting
E-P=ELISA (Peptide)
Reactivity Key:
Species cross-reactivity is determined by western blot. Species enclosed in parentheses are predicted to react based on 100% sequence homology.
Protocols
- 5465:
- ELISA Peptide*, Western Blotting
* Product-specific protocol.
Specificity / Sensitivity
Phospho-Tyrosine Mouse mAb (P-Tyr-100) (HRP Conjugate) is a high affinity antibody. ELISAs against a wide variety of phosphopeptides indicate that P-Tyr-100 binds phospho-Tyr in a manner largely independent of the surrounding amino acid sequence. 2D gel western blot analysis of pervanadate-treated cell extracts also shows that P-Tyr-100 interacts with a broad range of tyrosine-phosphorylated proteins. P-Tyr-100 does not cross-react with peptides containing phospho-Ser or phospho-Thr. (U.S. Patent No's.: 6,441,140; 6,982,318; 7,259,022; 7,344,714; U.S.S.N. 11,484,485; and all foreign equivalents.)
Source / Purification
Monoclonal antibody is produced by immunizing animals with phospho-tyrosine containing peptides.
Western Blotting
Western blot analysis of extracts from Jurkat and 3T3/NIH cells, untreated and treated with sodium vanadate (1 mM for 30 minutes), using Phospho-Tyrosine Mouse mAb (P-Tyr-100) (HRP Conjugate).
ELISA-Peptide
Signal-to-noise (S/N) values of phosphopeptide versus nonphosphopeptide signals using LumiGLO® and Peroxide Reagents (#7003).
Description
This Cell Signaling Technology® antibody is conjugated to the carbohydrate groups of horseradish peroxidase (HRP) via its amine groups. The HRP conjugated antibody is expected to exhibit the same species cross-reactivity as the unconjugated Phospho-Tyrosine Mouse mAb (P-Tyr-100) #9411.
Background
Tyrosine phosphorylation plays a key role in cellular signaling (1). Research studies have shown that in cancer, unregulated tyrosine kinase activity can drive malignancy and tumor formation by generating inappropriate proliferation and survival signals (2). Antibodies specific for phospho-tyrosine (3,4) have been invaluable reagents in these studies. The phospho-tyrosine monoclonal antibodies developed by Cell Signaling Technology are exceptionally sensitive tools for studying tyrosine phosphorylation and monitoring tyrosine kinase activity in high throughput drug discovery.
- Schlessinger, J. (2000) Cell 103, 211-25.
- Blume-Jensen, P. and Hunter, T. (2001) Nature 411, 355-65.
- Ward, S.G. et al. (1992) J Biol Chem 267, 23862-9.
- Glenney, J.R. et al. (1988) J Immunol Methods 109, 277-85.
Application References
Have you published research involving the use of our products? If so we'd love to hear about it. Please let us know!
Companion Products
- 9417 Phospho-Tyrosine Mouse mAb (P-Tyr-100) (Biotinylated)
- 9411 Phospho-Tyrosine Mouse mAb (P-Tyr-100)
- 9414 Phospho-Tyrosine Mouse mAb (P-Tyr-100) (Alexa Fluor® 488 Conjugate)
- 9415 Phospho-Tyrosine Mouse mAb (P-Tyr-100) (Alexa Fluor® 647 Conjugate)
- 9419 Phospho-Tyrosine Mouse mAb (P-Tyr-100) (Sepharose Bead Conjugate)
For Research Use Only. Not For Use In Diagnostic Procedures.
