Cell Signaling Technology

Product Pathways - Adhesion

BSP II Antibody #5468

Applications Reactivity Sensitivity MW (kDa) Source
W IP H M R Endogenous 82 Rabbit

Applications Key:  W=Western Blotting  IP=Immunoprecipitation
Reactivity Key:  H=Human  M=Mouse  R=Rat
Species cross-reactivity is determined by western blot. Species enclosed in parentheses are predicted to react based on 100% sequence homology.

Protocols

Specificity / Sensitivity

The BSP II Antibody detects endogenous levels of total BSP II protein.

Source / Purification

Polyclonal antibodies are produced by immunizing animals with a synthetic peptide corresponding to residues near the amino terminus of human BSP II protein. Antibodies are purified by protein A and peptide affinity chromatography.

Western Blotting

Western Blotting

Western blot analysis of extracts from MDA-MB-435, U-2 OS, and Saos-2 cells using BSP II Antibody.

Background

Bone sialoprotein II (BSP II) exists as a secreted protein in extracellular matrix of the skeleton (1-2). BSP II is part of the small integrin binding ligand N-linked glycoprotein (SIBLING) family and its role is crucial for cell to matrix adhesion as well as between two active protein complexes (3). Flexible binding capacity of BSP II also allows it to rapidly associate with various proteins, calcium, and mineralized phases of developing bone (3-5). BSP II plays a central role in the initiation of hydroxyapatite microcalcification of breast cancer cells that contributes to the pathogenesis of bone metastasis (4,6,7). Recent evidence suggests that the downregulation of BSP II may lead to the reduced formation of metastatic lesions and bone metastasis in a human breast cancer model (5).

  1. Papagerakis, P. et al. (2002) Bone 30, 377-85.
  2. Bellahcène, A. et al. (1994) Cancer Res 54, 2823-6.
  3. Fisher, L.W. et al. (2001) Biochem Biophys Res Commun 280, 460-5.
  4. Castronovo, V. and Bellahcene, A. (1998) Int J Oncol 12, 305-8.
  5. Adwan, H. et al. (2004) Cancer Gene Ther 11, 109-20.
  6. Diel, I.J. et al. (1999) Clin Cancer Res 5, 3914-9.
  7. Sharp, J.A. et al. (1999) Lab Invest 79, 869-77.

Application References

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Companion Products

For Research Use Only. Not For Use In Diagnostic Procedures.

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