Product Pathways - Cytoskeletal Signaling
Diap2 Antibody #5474
|5474S||100 µl (10 western blots)||---||In Stock||---|
|5474||carrier free and custom formulation / quantity||email request|
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|W||1:1000||Human, Mouse, Rat, Monkey||Endogenous||130||Rabbit|
Species cross-reactivity is determined by western blot.
Applications Key: W=Western Blotting, IP=Immunoprecipitation
Specificity / Sensitivity
Diap2 Antibody recognizes endogenous levels of total diap2 protein.
Source / Purification
Polyclonal antibodies are produced by immunizing animals with a synthetic peptide corresponding to residues near the amino terminus of human diap2 protein. Antibodies are purified by protein A and peptide affinity chromatography.
Formins are a family of large multidomain actin nucleation/polymerization proteins characterized by their catalytic FH2 domains. The mammalian diaphanous-related formin (mDia/diap) subfamily, including mDia1/diap1, mDia2/diap3 and mDia3/diap2, are effectors of Rho family small GTPases. In response to Rho, mDia/diap proteins are involved in the regulation of multiple cell functions including cytoskeletal dynamics, migration, adhesion, polarity and cell shape (reviewed in 1,2).
mDia1/diap1 is activated by GTP-bound Rho, leading to Rho-associated kinase (ROCK)-dependent stress fiber formation (3,4). Rho activation of mDia1 has also been shown to regulate serum response factor (SRF)-dependent transcription (5), and has been implicated in human cancer phenotypes such as ras-mediated transformation, metastasis and invasion (reviewed in 6).
mDia3/diap2, activated by the Rho family small GTPase cdc42, regulates the attachment of microtubules to the kinetochore during mitosis in mammalian cells (7).
Rho-dependent activation of mDia2/diap3 is important in assembly of the contractile ring during cytokinesis (8,9).
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- Chesarone, M.A. et al. (2010) Nat Rev Mol Cell Biol 11, 62-74.
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- Ishizaki, T. et al. (2001) Nat Cell Biol 3, 8-14.
- Copeland, J.W. and Treisman, R. (2002) Mol Biol Cell 13, 4088-99.
- Narumiya, S. et al. (2009) Cancer Metastasis Rev 28, 65-76.
- Yasuda, S. et al. (2004) Nature 428, 767-71.
- Watanabe, S. et al. (2010) Mol Biol Cell 21, 3193-204.
- Watanabe, S. et al. (2008) Mol Biol Cell 19, 2328-38.
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