Product Pathways - Companion Products
GST (91G1) Rabbit mAb (HRP Conjugate) #5475
| Applications | Reactivity | Sensitivity | Isotype |
|---|---|---|---|
| W | Transfected Only | Rabbit IgG |
Applications Key:
W=Western Blotting
Reactivity Key:
Species cross-reactivity is determined by western blot. Species enclosed in parentheses are predicted to react based on 100% sequence homology.
Protocols
- 5475:
- Western Blotting
Specificity / Sensitivity
GST (91G1) Rabbit mAb (HRP Conjugate) detects transfected GST fusion proteins.
Source / Purification
Monoclonal antibody is produced by immunizing animals with a GST fusion protein.
Western Blotting
Western blot analysis of extracts from COS-7 cells, either mock-transfected or transfected with a construct overexpressing GST-TrCP1, using GST (91G1) Rabbit mAb (HRP Conjugate).
Description
This Cell Signaling Technology antibody is conjugated to the carbohydrate groups of horseradish peroxidase (HRP) via its amine groups. The HRP conjugated antibody is expected to exhibit the same species cross-reactivity as the unconjugated GST (91G1) Rabbit mAb #2625.
Background
Epitope tags are useful for the labeling and detection of proteins using immunoblotting, immunoprecipitation, and immunostaining techniques. Because of their small size, they are unlikely to affect the tagged protein's biochemical properties.
Glutathione S-transferase (GST) is a widely used fusion partner, since it provides both an easily detectable Tag and a simple purification process with little effect on the biological function of the protein of interest. Numerous vectors containing GST-Tag have been developed for both prokaryotic and eukaryotic systems over the past decade (1-3).
- Guan, K.L. and Dixon, J.E. (1991) Anal Biochem 192, 262-7.
- Davies, A.H. et al. (1993) Biotechnology (N Y) 11, 933-6.
- Yu, J. et al. (1998) Mol Cell Biol 18, 1379-87.
Application References
Have you published research involving the use of our products? If so we'd love to hear about it. Please let us know!
Companion Products
- 7075 Anti-biotin, HRP-linked Antibody
- 2625 GST (91G1) Rabbit mAb
- 2624 GST (26H1) Mouse mAb
- 2622 GST Antibody
- 9962 Epitope Tag Antibody Sampler Kit
Rabbit monoclonal antibody is produced under license (granting certain rights including those under U. S. Patents No. 5,675,063 and7,429,487) from Epitomics, Inc.
For Research Use Only. Not For Use In Diagnostic Procedures.
