Product Pathways - Cytoskeletal Signaling
CD2AP (A599) Antibody #5478
PhosphoSitePlus® protein, site, and accession data: CD2AP
| Applications | Reactivity | Sensitivity | MW (kDa) | Source |
|---|---|---|---|---|
| W IP IF-IC | H M (Mk) | Endogenous | 80 | Rabbit |
Applications Key:
W=Western Blotting
IP=Immunoprecipitation
IF-IC=Immunofluorescence (Immunocytochemistry)
Reactivity Key:
H=Human
M=Mouse
Mk=Monkey
Species cross-reactivity is determined by western blot. Species enclosed in parentheses are predicted to react based on 100% sequence homology.
Protocols
Specificity / Sensitivity
CD2AP (A599) Antibody recognizes endogenous levels of total CD2AP protein.
Source / Purification
Polyclonal antibodies are produced by immunizing animals with a synthetic peptide corresponding to residues surrounding Ala599 of human CD2AP protein. Antibodies are purified by protein A and peptide affinity chromatography.
Western Blotting
Western blot analysis of extracts from various cell lines using CD2AP (A599) Antibody.
IF-IC
Confocal immunofluorescent analysis of A-431 cells using CD2AP (A599) Antibody (green). Actin filaments were labeled with DY-554 phalloidin (red). Blue pseudocolor = DRAQ5® #4084 (fluorescent DNA dye).
Background
CD2AP is a scaffolding protein that is thought to link membrane proteins to the cytoskeleton (1-3). It plays a role in formation of tight junctions in specialized cell types such as the slit diaphragm in the kidney glomerulus (4). CD2AP is also involved in the immunological synapse between CD2 expressing T cells and antigen presenting cells (5). It has been shown that interaction between CD2AP and other cytoskeletal proteins may regulate the endocytosis of EGFR (3).
- Kirsch, K.H. et al. (1999) Proc. Natl. Acad. Sci. USA 96, 6211-6216.
- Kirsch, K.H. et al. (2001) J. Biol. Chem. 276, 4957-4963.
- Lynch, D.K. et al. (2003) J. Biol. Chem. 278, 21805-21813.
- Kawachi, H. et al. (2006) Nephrology (Carlton) 11, 274-281.
- Hutchings, N.J. et al. (2003) J. Biol. Chem. 278, 22396-22403.
Application References
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For Research Use Only. Not For Use In Diagnostic Procedures.
