Cell Signaling Technology

Product Pathways - Ca / cAMP / Lipid Signaling

cPLA2 (D11H4) Rabbit mAb #5479

Applications Reactivity Sensitivity MW (kDa) Isotype
W IP H Endogenous 98 Rabbit IgG

Applications Key:  W=Western Blotting  IP=Immunoprecipitation
Reactivity Key:  H=Human
Species cross-reactivity is determined by western blot. Species enclosed in parentheses are predicted to react based on 100% sequence homology.

Protocols

Specificity / Sensitivity

cPLA2 (D11H4) Rabbit mAb detects endogenous levels of total cPLA2 protein.

Source / Purification

Monoclonal antibody is produced by immunizing animals with a synthetic peptide corresponding to residues surrounding Ala452 of human cPLA2 protein.

Western Blotting

Western Blotting

Western blot analysis of extracts from various cell lines using cPLA2 (D11H4) Rabbit mAb.

Background

Cytosolic phospholipase A2 (cPLA2) is a ubiquitously distributed enzyme that catalyzes the hydrolysis of the sn-2 acyl bond of glycerolipids to produce lysophospholipids and release arachidonic acid (1). cPLA2 has been implicated in diverse cellular responses such as mitogenesis, differentiation, inflammation and cytotoxicity (1). Calcium binding to the amino-terminal CalB domain of cPLA2 promotes the translocation of cPLA2 from cytosol to membrane, where cPLA2 cleaves arachidonic acid from natural membrane (2). Phosphorylation of cPLA2 by MAPK (p42/44 and p38) at Ser505 (3,4) and Ser727 (5) stimulates its catalytic activity.

  1. Leslie, C.C. (1997) J. Biol. Chem. 272, 16709-16712.
  2. Clark, J. D. et al. (1991) Cell 65, 1043-1051.
  3. Lin, L. L. et al. (1993) Cell 72, 269-278.
  4. Kramer, R.M. (1996) J. Biol. Chem. 271, 27723-27729.
  5. Hefner, Y. et al. (2000) J. Biol. Chem. 275, 37542-37551.

Application References

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Companion Products

For Research Use Only. Not For Use In Diagnostic Procedures.

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