Cell Signaling Technology

Product Pathways - Cytoskeletal Signaling

Phospho-Ezrin (Tyr353) (D66D3) Rabbit mAb #5484

Applications Reactivity Sensitivity MW (kDa) Isotype
W H (Mk) Endogenous 80 Rabbit IgG

Applications Key:  W=Western Blotting
Reactivity Key:  H=Human  Mk=Monkey
Species cross-reactivity is determined by western blot. Species enclosed in parentheses are predicted to react based on 100% sequence homology.

Protocols

Specificity / Sensitivity

Phospho-Ezrin (Tyr353) (D66D3) Rabbit mAb recognizes endogenous levels of ezrin protein only when phosphorylated at Tyr353. Based on protein sequences, the antibody is not expected to cross-react with radixin or moesin.

Source / Purification

Monoclonal antibody is produced by immunizing animals with a synthetic peptide corresponding to residues surrounding Tyr353 of human ezrin protein.

Western Blotting

Western Blotting

Western blot analysis of extracts from A-431 cells, untreated or treated with hEGF #8916 (100 ng/ml, 5 minutes), using Phospho-Ezrin (Tyr353) (D66D3) Rabbit mAb (upper) or Ezrin/Radixin/Moesin Antibody #3142 (lower).

Background

The ezrin, radixin, and moesin (ERM) proteins function as linkers between the plasma membrane and the actin cytoskeleton and are involved in cell adhesion, membrane ruffling, and microvilli formation (1). ERM proteins undergo intra or intermolecular interaction between their amino- and carboxy-terminal domains, existing as inactive cytosolic monomers or dimers (2). Phosphorylation at a carboxy-terminal threonine residue (Thr567 of ezrin, Thr564 of radixin, Thr558 of moesin) disrupts the amino- and carboxy-terminal association and may play a key role in regulating ERM protein conformation and function (3,4). Phosphorylation at Thr567 of ezrin is required for cytoskeletal rearrangements and oncogene-induced transformation (5). Ezrin is also phosphorylated at tyrosine residues upon growth factor stimulation. Phosphorylation of Tyr353 of ezrin transmits a survival signal during epithelial differentiation (6).

  1. Tsukita, S. and Yonemura, S. (1999) J. Biol. Chem. 274, 34507-34510.
  2. Mangeat, P. et al. (1999) Trends Cell Biol. 9, 187-192.
  3. Matsui, T. et al. (1998) J. Cell Biol. 140, 647-657.
  4. Gautreau, A. et al. (2000) J. Cell Biol. 150, 193-203.
  5. Tran Quang, C. et al. (2000) EMBO J. 19, 4565-4576.
  6. Gautreau, A. et al. (1999) Proc. Natl. Acad. Sci. USA 96, 7300-7305.

Application References

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For Research Use Only. Not For Use In Diagnostic Procedures.

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