Cell Signaling Technology

Product Pathways - Cytoskeletal Signaling

Diap1 Antibody #5486

Applications Reactivity Sensitivity MW (kDa) Source
W H Mk Endogenous 150 Rabbit

Applications Key:  W=Western Blotting
Reactivity Key:  H=Human  Mk=Monkey
Species cross-reactivity is determined by western blot. Species enclosed in parentheses are predicted to react based on 100% sequence homology.

Protocols

Specificity / Sensitivity

Diap1 Antibody recognizes endogenous levels of total Diap1 protein.

Source / Purification

Polyclonal antibodies are produced by immunizing animals with a synthetic peptide corresponding to residues near the carboxy terminus of human Diap1 protein. Antibodies are purified by protein A and peptide affinity chromatography.

Western Blotting

Western Blotting

Western blot analysis of extracts from various cell lines using Diap1 Antibody.

Background

Formins are a family of large multidomain actin nucleation/polymerization proteins characterized by their catalytic FH2 domains. The mammalian diaphanous-related formin (mDia/diap) subfamily, including mDia1/diap1, mDia2/diap3 and mDia3/diap2, are effectors of Rho family small GTPases. In response to Rho, mDia/diap proteins are involved in the regulation of multiple cell functions including cytoskeletal dynamics, migration, adhesion, polarity and cell shape (reviewed in 1,2).mDia1/diap1 is activated by GTP-bound Rho, leading to Rho-associated kinase (ROCK)-dependent stress fiber formation (3,4). Rho activation of mDia1 has also been shown to regulate serum response factor (SRF)-dependent transcription (5), and has been implicated in human cancer phenotypes such as ras-mediated transformation, metastasis and invasion (reviewed in 6).mDia3/diap2, activated by the Rho family small GTPase cdc42, regulates the attachment of microtubules to the kinetochore during mitosis in mammalian cells (7).Rho-dependent activation of mDia2/diap3 is important in assembly of the contractile ring during cytokinesis (8,9).

  1. Schönichen, A. and Geyer, M. (2010) Biochim Biophys Acta 1803, 152-63.
  2. Chesarone, M.A. et al. (2010) Nat Rev Mol Cell Biol 11, 62-74.
  3. Watanabe, N. et al. (1999) Nat Cell Biol 1, 136-43.
  4. Ishizaki, T. et al. (2001) Nat Cell Biol 3, 8-14.
  5. Copeland, J.W. and Treisman, R. (2002) Mol Biol Cell 13, 4088-99.
  6. Narumiya, S. et al. (2009) Cancer Metastasis Rev 28, 65-76.
  7. Yasuda, S. et al. (2004) Nature 428, 767-71.
  8. Watanabe, S. et al. (2010) Mol Biol Cell 21, 3193-204.
  9. Watanabe, S. et al. (2008) Mol Biol Cell 19, 2328-38.

Application References

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Companion Products

For Research Use Only. Not For Use In Diagnostic Procedures.

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