Cell Signaling Technology

Product Pathways - Motif Antibodies

Phospho-MAPK/CDK Substrates (PXS*P or S*PXR/K) (34B2) Rabbit mAb (Sepharose Bead Conjugate) #5501

Applications Reactivity Sensitivity Isotype
IP Endogenous Rabbit IgG

Applications Key:  IP=Immunoprecipitation
Reactivity Key:
Species cross-reactivity is determined by western blot. Species enclosed in parentheses are predicted to react based on 100% sequence homology.

Protocols

Specificity / Sensitivity

Phospho-MAPK/CDK Substrates (PXS*P or S*PXR/K) (34B2) Rabbit mAb (Sepharose Bead Conjugate) detects phospho-serine in a PXS*P or S*PXR/K motif, as well as a PXS*PXR/K motif. The antibody is phospho-specific, and does not react with phospho-threonine- or phospho-tyrosine-containing peptides/proteins. (U.S. Patent No's.: 6,441,140; 6,982,318; 7,259,022; 7,344,714; U.S.S.N. 11,484,485; and all foreign equivalents.)

Source / Purification

Monoclonal antibody is produced by immunizing animals with synthetic phospho-MAPK/CDK substrate peptides.

IP

IP

Immunoprecipitation of Jurkat cell extracts, untreated (-) or treated with Calyculin A #9902 (+), using Phospho-MAPK/CDK Substrates (PXS*P or S*PXR/K) (34B2) Rabbit mAb (Sepharose Bead Conjugate) and Rabbit (DA1E) mAb IgG XP® Isotype Control (Sepharose Bead Conjugate) #3423. The western blot was probed using Phospho-MAPK/CDK Substrates (PXS*P or S*PXR/K) (34B2) Rabbit mAb #2325. Mouse Anti-rabbit IgG (Conformation Specific) (L27A9) mAb (HRP Conjugate) #5127 was used as a secondary antibody.

Description

This Cell Signaling Technology antibody is immobilized via covalent binding of primary amino groups to N-hydroxysuccinimide (NHS)-activated sepharose beads. Phospho-MAPK/CDK Substrates (PXS*P or S*PXR/K) (34B2) Rabbit mAb (Sepharose Bead Conjugate) is useful for the immunoprecipitation of Phospho-MAPK/CDK Substrates (PXS*P or S*PXR/K). The antibody is expected to exhibit the same species cross-reactivity as the unconjugated Phospho-MAPK/CDK Substrates (PXS*P or S*PXR/K) (34B2) Rabbit mAb #2325.

Background

The MAPK and CDK families of serine/threonine protein kinases play important roles in proliferation and cell cycle control. These kinases phosphorylate threonine or serine followed by a proline residue (1-3). MAPK phosphorylates substrates with the consensus sequence PX(S/T)P, and CDKs phosphorylate substrates containing the consensus sequence (S/T)PXR/K. Cell Signaling Technology has developed antibodies that bind to phospho-threonine followed by proline, motifs PXS*/T*P and/or S*PXR/K, for use in the study and discovery of new MAPK and CDK substrates (4,5).

  1. Cross, T.G. et al. (2000) Exp Cell Res 256, 34-41.
  2. Reynolds, C.H. et al. (2000) J Neurochem 74, 1587-95.
  3. Seger, R. and Krebs, E.G. (1995) FASEB J 9, 726-35.
  4. Holmes, J.K. and Solomon, M.J. (1996) J Biol Chem 271, 25240-6.
  5. Songyang, Z. et al. (1996) Mol Cell Biol 16, 6486-93.

Application References

Have you published research involving the use of our products? If so we'd love to hear about it. Please let us know!

Rabbit monoclonal antibody is produced under license (granting certain rights including those under U. S. Patents No. 5,675,063 and 7,429,487) from Epitomics, Inc.

For Research Use Only. Not For Use In Diagnostic Procedures.

Products