Cell Signaling Technology

Product Pathways - Cytoskeletal Signaling

Occludin Antibody #5506

Applications Reactivity Sensitivity MW (kDa) Source
W H Mk (Dg) Endogenous 65 Rabbit

Applications Key:  W=Western Blotting
Reactivity Key:  H=Human  Mk=Monkey  Dg=Dog
Species cross-reactivity is determined by western blot. Species enclosed in parentheses are predicted to react based on 100% sequence homology.

Protocols

Specificity / Sensitivity

Occludin Antibody detects endogenous levels of total occludin protein.

Source / Purification

Polyclonal antibodies are produced by immunizing animals with a synthetic peptide corresponding to the carboxy terminus of human occludin protein.

Western Blotting

Western Blotting

Western blot analysis of extracts from Hep-G2, HUVE and A-431 cells using Occludin Antibody.

Background

Tight junctions, or zona occludens, form a continuous barrier to fluids across the epithelium and endothelium. They function in regulation of paracellular permeability and in the maintenance of cell polarity, blocking the movement of transmembrane proteins between the apical and the basolateral cell surfaces (reviewed in 1). Tight junctions are composed of claudin and occludin transmembrane proteins, which join the junctions to the cytoskeleton (1,2). Occludin is thought to be important in the assembly and maintenance of tight junctions. Differential phosphorylation of occludin at various residues may regulate its interaction with other tight junction proteins such as ZO-1 (3). VEGF-induced phosphorylation of occludin regulates tight junction stability and vascular permeability (4). Expression of occludin as well as claudin1 is required for infection of liver cells by hepatitis C virus (HCV) (5).

  1. Shin, K. et al. (2006) Annu Rev Cell Dev Biol 22, 207-35.
  2. Oliveira, S.S. and Morgado-Díaz, J.A. (2007) Cell Mol Life Sci 64, 17-28.
  3. Rao, R. (2009) Ann N Y Acad Sci 1165, 62-8.
  4. Murakami, T. et al. (2009) J Biol Chem 284, 21036-46.
  5. Ploss, A. et al. (2009) Nature 457, 882-6.

Application References

Have you published research involving the use of our products? If so we'd love to hear about it. Please let us know!

Companion Products

This product is intended for research purposes only. The product is not intended to be used for therapeutic or diagnostic purposes in humans or animals.

Products