Cell Signaling Technology

Product Pathways - Neuroscience

Plexin A3 (D2G12) Rabbit mAb #5512

Applications Reactivity Sensitivity MW (kDa) Isotype
W IP H Endogenous 207 Rabbit IgG

Applications Key:  W=Western Blotting  IP=Immunoprecipitation
Reactivity Key:  H=Human
Species cross-reactivity is determined by western blot. Species enclosed in parentheses are predicted to react based on 100% sequence homology.

Protocols

Specificity / Sensitivity

Plexin A3 (D2G12) Rabbit mAb detects endogenous levels of total Plexin A3 protein. Plexin A3 (D2G12) Rabbit mAb may cross-react with other family members.

Source / Purification

Monoclonal antibody is produced by immunizing animals with a synthetic peptide surrounding Val440 of human Plexin A3.

Western Blotting

Western Blotting

Western blot analysis of extracts from human cerebellum, A204 and MCF-7 cells using Plexin A3 (D2G12) Rabbit mAb.

Background

Class 3 secreted semaphorin (Sema3A) is a chemorepellent that acts upon a wide variety of axons. As such, it induces a dramatic redistribution and depolymerization of actin filaments that results in growth cone collapse. Plexins are single membrane-spanning signaling proteins encompassing Plexin A1, A2, A3, and A4. Plexins form a complex with neuropilin-1 and -2 and the cell adhesion protein L1 to form a functional semaphorin receptor (1,2). The GTPase Rnd1 binds to the cytoplasmic domain of Plexin A1 to trigger cytoskeletal collapse. In contrast, the GTPase RhoD blocks Rnd1-mediated Plexin A1 activation and repulsion of sympathetic axons by Sema3A (3).

Both Plexin A3 and A4 mediate the responses to class 3 semaphorins in sensory and sympathetic neurons. In particular, Plexin A4 is responsible for signaling of Sema 3A via neuropilin-1, while Plexin A3 is responsible for signaling of Sema 3F via neuropilin-2 (4).

  1. Pasterkamp, R.J. and Kolodkin, A.L. (2003) Curr Opin Neurobiol 13, 79-89.
  2. Takahashi, T. and Strittmatter, S.M. (2001) Neuron 29, 429-39.
  3. Zanata, S.M. et al. (2002) J Neurosci 22, 471-7.
  4. Yaron, A. et al. (2005) Neuron 45, 513-23.

Application References

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Companion Products

For Research Use Only. Not For Use In Diagnostic Procedures.

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