Product Pathways - MAPK Signaling
JIP4/SPAG9 (D72F4) XP® Rabbit mAb #5519
|5519S||100 µl (10 western blots)||---||In Stock||---|
|5519P||40 µl (4 western blots)||---||In Stock||---|
|5519||carrier free and custom formulation / quantity||email request|
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|W||1:1000||Human, Mouse, Rat, Monkey||Endogenous||190||Rabbit IgG|
Species cross-reactivity is determined by western blot.
Applications Key: W=Western Blotting, IP=Immunoprecipitation, IHC-P=Immunohistochemistry (Paraffin), IF-IC=Immunofluorescence (Immunocytochemistry), F=Flow Cytometry
Specificity / Sensitivity
JIP4/SPAG9 (D72F4) XP® Rabbit mAb recognizes endogenous levels of total JIP4 protein. This antibody will detect known splice variants of JIP4 (SPAG9, JLP) but is not predicted to cross-react with other JIP family members.
Source / Purification
Monoclonal antibody is produced by immunizing animals with a synthetic peptide corresponding to a region surrounding Leu237 of human JIP4 protein.
Western blot analysis of extracts from various tissues and cell lines using JIP4/SPAG9 (D72F4) XP® Rabbit mAb.
Immunohistochemical analysis of paraffin-embedded human ovarian carcioma using JIP4/SPAG9 (D72F4) XP® Rabbit mAb in the presence of control peptide (left) or antigen-specific peptide (right).
Immunohistochemical analysis of paraffin-embedded human lung adenocarcioma using JIP4/SPAG9 (D72F4) XP® Rabbit mAb.
Flow cytometric analysis of NIH/3T3 cells using JIP4/SPAG9 (D72F4) XP® Rabbit mAb (blue) compared to a nonspecific negative control antibody (red).
Confocal immunofluorescent analysis of HeLa cells untreated (left) or nocodazole-treated (right) using JIP4/SPAG9 (D72F4) XP® Rabbit mAb (green). Blue pseudocolor= DRAQ5® #4084 (fluorescent DNA dye).
Confocal immunofluorescent analysis of A172 cells using JIP4/SPAG9 (D72F4) XP® Rabbit mAb (green). Blue pseudocolor= DRAQ5® #4084 (fluorescent DNA dye).
JNK-Interacting Proteins (JIPs), as their name implies, coordinate c-Jun N-terminal Kinase (JNK) signaling by acting as scaffolds for components of the JNK signaling cascade (1). JIPs localize and promote JNK activation in response to stress by amalgamating and co-localizing upstream kinases and downstream effectors in the stress-kinase pathway analogous to the mechanism by which AKAPs orchestrate PKA signaling. JIPs bind to an array of MAPKs and other signaling proteins, including the mixed-lineage kinases, MKK7, p38α MAPK, JNK1-3, Max, Myc, NF-κB, LRRK2, and others (1-4).
There are four known JIPs, JIP1-4, of which JIP1 and JIP2 share extensive sequence homology and domain structure. JIP1 and JIP2 are mainly expressed in neurons, testis and in β pancreatic cells, where they have been implicated in cellular responses to metabolic stress, the development of diabetes, and post-traumatic brain damage (5-7). Although architecturally distinct from JIP1 and JIP2, JIP3 and JIP4 share some overlapping functions and are more broadly expressed. JIP4, encoded by the SPAG9 (sperm-associated antigen-9) gene, is a homooligomer that binds to and coordinates the activation of numerous components of the stress-activated kinase cascade including MEK4, MEKK3, p38α MAPK, and JNK1-3 (3,8). However, unlike the other JIP members, JIP4 does not appear to activate JNK directly, instead favoring stimulation of p38 MAPK signaling events in response to cellular stress (3,9).
In addition to mediating stress responses, JIP4 (or its splice variant, JLP) has also been shown to interact with ARF6 and PIKfyve, thus regulating microtubule-based endosomal trafficking (10,11). There are extensive reports indicating that JIP4 is phosphorylated in response to stress (UV damage etc.) but it is unclear what effect, if any, this has on its function, localization, or binding properties (12-15).
- Jagadish, N. et al. (2005) Biochem J 389, 73-82.
- Bouwmeester, T. et al. (2004) Nat Cell Biol 6, 97-105.
- Kelkar, N. et al. (2005) Mol Cell Biol 25, 2733-43.
- Hsu, C.H. et al. (2010) Neurodegener Dis 7, 68-75.
- Willoughby, E.A. et al. (2003) J Biol Chem 278, 10731-6.
- Koushika, S.P. (2008) Bioessays 30, 10-4.
- Beeler, N. et al. (2009) Brain Res Bull 80, 274-81.
- Jagadish, N. et al. (2005) Biochem J 389, 73-82.
- Brancho, D. et al. (2003) Genes Dev 17, 1969-78.
- Ikonomov, O.C. et al. (2009) J Biol Chem 284, 3750-61.
- Montagnac, G. et al. (2009) Curr Biol 19, 184-95.
- Olsen, J.V. et al. (2006) Cell 127, 635-48.
- Molina, H. et al. (2007) Proc Natl Acad Sci U S A 104, 2199-204.
- Matsuoka, S. et al. (2007) Science 316, 1160-6.
- Dephoure, N. et al. (2008) Proc Natl Acad Sci U S A 105, 10762-7.
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Cell Signaling Technology® is a trademark of Cell Signaling Technology, Inc.