Cell Signaling Technology

Product Pathways - Cell Cycle / Checkpoint

PITSLRE/CDK11 (D88B3) Rabbit mAb #5524

Applications Reactivity Sensitivity MW (kDa) Isotype
W IP IF-IC H M Mk (R) Endogenous 110 Rabbit IgG

Applications Key:  W=Western Blotting  IP=Immunoprecipitation  IF-IC=Immunofluorescence (Immunocytochemistry)
Reactivity Key:  H=Human  M=Mouse  R=Rat  Mk=Monkey
Species cross-reactivity is determined by western blot. Species enclosed in parentheses are predicted to react based on 100% sequence homology.

Protocols

Specificity / Sensitivity

PITSLRE/CDK11 (D88B3) Rabbit mAb recognizes endogenous levels of total PITSLRE/CDK11 protein. This antibody will detect the full-length (CDK11p110) and is predicted to detect the alternate transcript (CDK11p58) of PITSLRE/CDK11. The antibody is predicted to detect both PITSLREA/CDK11B and PITSLREB/CDK11A.

Source / Purification

Monoclonal antibody is produced by immunizing animals with a synthetic peptide corresponding to residues surrounding Met734 of human PITSLRE/CDK11 protein.

Western Blotting

Western Blotting

Western blot analysis of extracts from various cell lines using PITSLRE/CDK11 (D88B3) Rabbit mAb.

IF-IC

IF-IC

Confocal immunofluorescent analysis of A-431 cells (left) and UACC62 cells (right) using PITSLRE/CDK11 (D88B3) Rabbit mAb (green). Actin filaments were labeled with DY-554 phalloidin (red).

Background

PITSLRE, alternatively known as cell division kinase 11 (CDK11), is the result of duplication of the CDK11 gene (1). CDK11A and CDK11B encode nearly identical serine/threonine protein kinases, PITSLREB and PITSLREA respectively, both belonging to the p34CDC2 family of protein kinases (2). Full-length PITSLRE/CDK11 (commonly referred to as CDK11p110) is expressed ubiquitously throughout the cell cycle whereas a smaller, alternate transcript (CDK11p58), the result of internal ribosomal entry, is expressed only during the G2/M transition where it promotes centrosome maturation and spindle formation (3-5). During induction of apoptosis by Fas or TNF, or anoikis, PITSLRE/CDK11 is cleaved by caspases to generate p110C, an approximately 46 kDa protein that contains the catalytically active kinase domain of PITSLRE/CDK11 that interacts with and inhibits p21-activated kinase (PAK1) activity (6-8). Full length PITSLRE/CDK11 (CDK11p110) appears to participate in pre-mRNA splicing events. This is demonstrated by the observation that CDK11p110 interacts with numerous splicing factors including RNPS1, 9G8/SRSF7 and cyclin L and that CDK11p110 can phosphorylate and inhibit the splicing activity of 9G8/SRSF7 (9-11).

  1. Gururajan, R. et al. (1998) Genome Res 8, 929-39.
  2. Xiang, J. et al. (1994) J Biol Chem 269, 15786-94.
  3. Cornelis, S. et al. (2000) Mol Cell 5, 597-605.
  4. Petretti, C. et al. (2006) EMBO Rep 7, 418-24.
  5. Wilker, E.W. et al. (2007) Nature 446, 329-32.
  6. Chen, S. et al. (2003) J Biol Chem 278, 20029-36.
  7. Lahti, J.M. et al. (1995) Mol Cell Biol 15, 1-11.
  8. Ariza, M.E. et al. (1999) J Biol Chem 274, 28505-13.
  9. Hu, D. et al. (2003) J Biol Chem 278, 8623-9.
  10. Loyer, P. et al. (1998) J Cell Sci 111 ( Pt 11), 1495-506.
  11. Dickinson, L.A. et al. (2002) J Biol Chem 277, 25465-73.

Application References

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For Research Use Only. Not For Use In Diagnostic Procedures.

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