Cell Signaling Technology

Product Pathways - Tyrosine Kinase / Adaptors

Phospho-PZR (Tyr263) Antibody #5543

Applications Reactivity Sensitivity MW (kDa) Source
W IP H M R B Endogenous 30-50 Rabbit

Applications Key:  W=Western Blotting  IP=Immunoprecipitation
Reactivity Key:  H=Human  M=Mouse  R=Rat  B=Bovine
Species cross-reactivity is determined by western blot. Species enclosed in parentheses are predicted to react based on 100% sequence homology.

Protocols

Specificity / Sensitivity

Phospho-PZR (Tyr263) Antibody recognizes endogenous levels of PZR protein only when phosphorylated at Tyr263. This antibody does not cross-react with other tyrosine kinase proteins.

Source / Purification

Polyclonal antibodies are produced by immunizing animals with a synthetic peptide corresponding to residues surrounding Tyr263 of human PZR protein. Antibodies are purified by protein A and peptide affinity chromatography.

Western Blotting

Western Blotting

Western blot analysis of extracts from BAEC cells, untreated or H2O2-treated (0.3 mM for 30 min), using Phospho-PZR (Tyr263) Antibody (upper) and total PZR Antibody # 4157 (lower).

Background

PZR (Protein zero related) is an immunoglobulin superfamily protein that specifically binds the tyrosine phosphatase SHP-2 through its intracellular immunoreceptor tyrosine-based inhibitory motifs (ITIMs) (1,2). PZR is phosphorylated by c-Src, c-Fyn, c-Lyn, Csk, and c-Abl (3). PP1, a Src family kinase inhibitor, inhibits PZR phosphorylation (4,5). There are three alternatively spliced isoforms, designated as PZR, PZRa, and PZRb; both PZRa and PZRb lack ITIMs (6,7). PZR is the main receptor of ConA and has an important role in cell signaling via c-Src (4). PZR is expressed in many cell types and is localized to cell contacts and intracellular granules in BAECs and mesothelioma (REN) cells. PZR has been implicated as a cell adhesion protein that may be involved in SHP-2-dependent signaling at interendothelial cell contacts (3). Hypertyrosine phosphorylation of PZR was observed during embryogenesis in a mouse model of Noonan syndrome (8).

  1. Zhao, Z.J. and Zhao, R. (1998) J Biol Chem 273, 29367-72.
  2. Zhao, R. and Zhao, Z.J. (2000) J Biol Chem 275, 5453-9.
  3. Kusano, K. et al. Endothelium 15, 127-36.
  4. Zhao, R. et al. (2002) J Biol Chem 277, 7882-8.
  5. Zhao, R. et al. (2003) J Biol Chem 278, 42893-8.
  6. Zannettino, A.C. et al. (2003) Biochem J 370, 537-49.
  7. Zhao, R. and Zhao, Z.J. (2003) Biochem Biophys Res Commun 303, 1028-33.
  8. Eminaga, S. and Bennett, A.M. (2008) J Biol Chem 283, 15328-38.

Application References

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For Research Use Only. Not For Use In Diagnostic Procedures.

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