Product Pathways - Translational Control
hnRNP A0 (D8A3) XP® Rabbit mAb #5545
PhosphoSitePlus® protein, site, and accession data: hnRNP A0
| Applications | Reactivity | Sensitivity | MW (kDa) | Isotype |
|---|---|---|---|---|
| W IP IF-IC F | H M R Mk | Endogenous | 32, 34 | Rabbit IgG |
Applications Key:
W=Western Blotting
IP=Immunoprecipitation
IF-IC=Immunofluorescence (Immunocytochemistry)
F=Flow Cytometry
Reactivity Key:
H=Human
M=Mouse
R=Rat
Mk=Monkey
Species cross-reactivity is determined by western blot. Species enclosed in parentheses are predicted to react based on 100% sequence homology.
Protocols
Specificity / Sensitivity
hnRNP A0 (D8A3) XP® Rabbit mAb recognizes endogenous levels of total hnRNP A0 protein. This antibody does not cross-react with other hnRNP family members.
Source / Purification
Monoclonal antibody is produced by immunizing animals with a synthetic peptide corresponding to residues surrounding Leu114 of human hnRNP A0 protein.
Western Blotting
Western blot analysis of extracts from various cell lines using hnRNP A0 (D8A3) XP® Rabbit mAb.
Western Blotting
Western blot analysis of extracts from HeLa cells, transfected with SignalSilence® Control siRNA (Unconjugated) #6568 (-) or SignalSilence® hnRNP A0 siRNA I #6503 (+), using hnRNP A0 (D8A3) XP® Rabbit mAb (upper) and β-Actin (13E5) Rabbit mAb #4970 (lower).
Flow Cytometry
Flow cytometric analysis of HeLa cells using hnRNP A0 (D8A3) XP® Rabbit mAb (blue) compared to a nonspecific negative control antibody (red).
IF-IC
Confocal immunofluorescent analysis of HeLa cells using hnRNP A0 (D8A3) XP® Rabbit mAb (green). Actin filaments were labeled with DY-554 phalloidin (red).
Background
Heterogeneous nuclear ribonucleoprotein A0 (hnRNP A0) is a member of the hnRNP A/B family of related RNA binding proteins that bind pre-mRNA and are involved in the processing, metabolism, and transport of nuclear pre-mRNA transcripts (1). The A/B subfamily of hnRNP includes A1, A2/B1, A3, and A0. hnRNP A0 is phosphorylated at Ser84 by MAPKAPK-2 in response to LPS treatment in mouse macrophage cells, which might play a key role in stimulating translation of the TNF-α message (2).
Application References
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For Research Use Only. Not For Use In Diagnostic Procedures.
