Product Pathways - Translational Control
hnRNP A0 (D8A3) XP® Rabbit mAb #5545
|5545S||100 µl (10 western blots)||---||In Stock||---|
|5545P||40 µl (4 western blots)||---||In Stock||---|
|5545||carrier free and custom formulation / quantity||email request|
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|W||1:1000||Human, Mouse, Rat, Monkey||Endogenous||32, 34||Rabbit IgG|
Species cross-reactivity is determined by western blot.
Applications Key: W=Western Blotting, IP=Immunoprecipitation, IF-IC=Immunofluorescence (Immunocytochemistry), F=Flow Cytometry
Specificity / Sensitivity
hnRNP A0 (D8A3) XP® Rabbit mAb recognizes endogenous levels of total hnRNP A0 protein. This antibody does not cross-react with other hnRNP family members.
Source / Purification
Monoclonal antibody is produced by immunizing animals with a synthetic peptide corresponding to residues surrounding Leu114 of human hnRNP A0 protein.
Western blot analysis of extracts from various cell lines using hnRNP A0 (D8A3) XP® Rabbit mAb.
Western blot analysis of extracts from HeLa cells, transfected with SignalSilence® Control siRNA (Unconjugated) #6568 (-) or SignalSilence® hnRNP A0 siRNA I #6503 (+), using hnRNP A0 (D8A3) XP® Rabbit mAb (upper) and β-Actin (13E5) Rabbit mAb #4970 (lower).
Flow cytometric analysis of HeLa cells using hnRNP A0 (D8A3) XP® Rabbit mAb (blue) compared to a nonspecific negative control antibody (red).
Heterogeneous nuclear ribonucleoprotein A0 (hnRNP A0) is a member of the hnRNP A/B family of related RNA binding proteins that bind pre-mRNA and are involved in the processing, metabolism, and transport of nuclear pre-mRNA transcripts (1). The A/B subfamily of hnRNP includes A1, A2/B1, A3, and A0. hnRNP A0 is phosphorylated at Ser84 by MAPKAPK-2 in response to LPS treatment in mouse macrophage cells, which might play a key role in stimulating translation of the TNF-α message (2).
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For Research Use Only. Not For Use In Diagnostic Procedures.
XP® is a trademark of Cell Signaling Technology, Inc.
Cell Signaling Technology® is a trademark of Cell Signaling Technology, Inc.