Product Pathways - Translational Control
DDX4 (2F9H5) Mouse mAb #5555
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PhosphoSitePlus® protein, site, and accession data: DDX4
| Applications | Reactivity | Sensitivity | MW (kDa) | Isotype |
|---|---|---|---|---|
| W IP | H | Endogenous | 80 | Mouse IgG1 |
Applications Key:
W=Western Blotting
IP=Immunoprecipitation
Reactivity Key:
H=Human
Species cross-reactivity is determined by western blot. Species enclosed in parentheses are predicted to react based on 100% sequence homology.
Protocols
Specificity / Sensitivity
DDX4 (2F9H5) Mouse mAb detects endogenous levels of total DDX4 protein.
Source / Purification
Monoclonal antibody is produced by immunizing animals with a purified recombinant fragment of human DDX4.
Western Blotting
Western blot analysis of extract from human testis tissue using DDX4 (2F9H5) Mouse mAb.
Background
DDX4 is an ATP-dependent DEAD-box RNA helicase found in the chromatoid body of the germ cells (1). This enzyme is specific to germ cells and is necessary for germ cell development (2). Mouse DDX4 was shown to interact with Dicer, suggesting a role in microRNA-mediated RNA silencing (1).
- Kotaja, N. et al. (2006) Proc Natl Acad Sci USA 103, 2647-52.
- Albamonte, M.S. et al. (2008) Hum Reprod 23, 1895-901.
Application References
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For Research Use Only. Not For Use In Diagnostic Procedures.
