Cell Signaling Technology

Product Pathways - Metabolism

DLST Antibody #5556

Applications Reactivity Sensitivity MW (kDa) Source
W IP H M R Mk Endogenous 50 Rabbit

Applications Key:  W=Western Blotting  IP=Immunoprecipitation
Reactivity Key:  H=Human  M=Mouse  R=Rat  Mk=Monkey
Species cross-reactivity is determined by western blot. Species enclosed in parentheses are predicted to react based on 100% sequence homology.

Protocols

Specificity / Sensitivity

DLST Antibody detects endogenous levels of total DLST protein.

Source / Purification

Polyclonal antibodies are produced by immunizing animals with a synthetic peptide corresponding to residues surrounding Pro188 of human DLST protein. Antibodies are purified by protein A and peptide affinity chromatography.

Western Blotting

Western Blotting

Western blot analysis of extracts from various cell lines using DLST Antibody.

Background

α-ketoglutarate dehydrogenase complex is a rate-regulating enzyme in the Krebs Cycle (1). Dihydrolipoamide succinyltransferase (DLST) is a key subunit in this complex (2). Reduction of DLST increases reactive oxygen species production, suggesting its role in oxidative stress (2). Research has shown that deficiency of DLST in mice is linked to increased oxidative stress in mitochondria, a process that may be involved in the pathogenesis of Alzheimer's disease (2).

  1. Ohta, S. and Ohsawa, I. (2006) J Alzheimers Dis 9, 155-66.
  2. Dumont, M. et al. (2009) Free Radic Biol Med 47, 1019-27.

Application References

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Companion Products

For Research Use Only. Not For Use In Diagnostic Procedures.

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