Cell Signaling Technology

Product Pathways - Neuroscience

DJ-1 (D21E11) Rabbit mAb #5560

Applications Reactivity Sensitivity MW (kDa) Isotype
W IP H Endogenous 22 Rabbit IgG

Applications Key:  W=Western Blotting  IP=Immunoprecipitation
Reactivity Key:  H=Human
Species cross-reactivity is determined by western blot. Species enclosed in parentheses are predicted to react based on 100% sequence homology.

Protocols

Specificity / Sensitivity

DJ-1 (D21E11) Rabbit mAb detects endogenous levels of total DJ-1 protein. DJ-1 (D21E11) Rabbit mAb is recommended to detect human samples but may also detect mouse and rat samples weakly.

Source / Purification

Monoclonal antibody is produced by immunizing animals with a synthetic peptide corresponding to residues surrounding Gly150 of human DJ-1 protein.

Western Blotting

Western Blotting

Western blot analysis of extracts from various cell lines and tissue using DJ-1 (D21E11) Rabbit mAb.

Background

Parkinson's disease (PD) is characterized by the presence of Lewy bodies (intracellular inclusions) and by the loss of dopaminergic neurons. Research studies have shown that mutations in α-synuclein, Parkin, and DJ-1 are linked to PD (1). α-synuclein is a major component of the aggregates found in Lewy bodies. Parkin is involved in protein degradation through the ubiquitin-proteasome pathway, and investigators have shown that mutations in Parkin cause early onset of PD (1). Loss-of-function mutations in DJ-1 cause early onset of PD, but DJ-1 is associated with multiple functions: it cooperates with Ras to increase cell transformation, it positively regulates transcription of the androgen receptor, and it may function as an indicator of oxidative stress (2-5). Dopamine D2 receptor-mediated functions are greatly impaired in DJ-1 (-/-) mice, resulting in reduced long-term depression (6).

  1. Borrelli, E. (2005) Neuron 45, 479-81.
  2. Bonifati, V. et al. (2003) Science 299, 256-9.
  3. Nagakubo, D. et al. (1997) Biochem. Biophys. Res. Commun. 231, 509-13.
  4. Takahashi, K. et al. (2001) J. Biol. Chem. 276, 37556-63.
  5. Mitsumoto, A. and Nakagawa, Y. (2001) Free Radic. Res. 35, 885-93.
  6. Goldberg, M.S. et al. (2005) Neuron 45, 489-96.

Application References

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Companion Products

For Research Use Only. Not For Use In Diagnostic Procedures.

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