Cell Signaling Technology
XP Monoclonal Antibody

Product Pathways - Neuroscience

β3-Tubulin (D71G9) XP® Rabbit mAb #5568

Applications Reactivity Sensitivity MW (kDa) Isotype
W IP IF-F IF-IC H M R Endogenous 55 Rabbit IgG

Applications Key:  W=Western Blotting  IP=Immunoprecipitation  IF-F=Immunofluorescence (Frozen)  IF-IC=Immunofluorescence (Immunocytochemistry)
Reactivity Key:  H=Human  M=Mouse  R=Rat
Species cross-reactivity is determined by western blot. Species enclosed in parentheses are predicted to react based on 100% sequence homology.

Protocols

Specificity / Sensitivity

β3-Tubulin (D71G9) XP® Rabbit mAb detects endogenous levels of total β3-tubulin protein. This antibody does not cross-react with tubulin isoforms expressed in non-neuronal cells. This clone is similar to TUJ1.

Source / Purification

Monoclonal antibody is produced by immunizing animals with a synthetic peptide corresponding to the carboxy terminus of human β3-tubulin protein.

Western Blotting

Western Blotting

Western blot analysis of HeLa and human cerebellum using β3-Tubulin (D71G9) XP® Rabbit mAb.

IF-IC

IF-IC

Confocal immunofluorescent analysis of P19 cells that were differentiated with retinoic acid, using β3-Tubulin (D71G9) XP® Rabbit mAb (green). Actin filaments were labeled with DY-554 phalloidin (red). Blue pseudocolor = DRAQ5 #4084® (fluorescent DNA dye).

IF-F

IF-F

Confocal immunofluorescent analysis of mouse cerebellum using β3-Tubulin (D71G9) XP® Rabbit mAb (green) and Tau (Tau46) Mouse mAb #4019 (red). Blue pseudocolor = DRAQ5® #4084 (fluorescent DNA dye).


Background

The cytoskeleton consists of three types of cytosolic fibers: microtubules, microfilaments (actin filaments), and intermediate filaments. Globular tubulin subunits comprise the microtubule building block, with α/β-tubulin heterodimers forming the tubulin subunit common to all eukaryotic cells. γ-tubulin is required to nucleate polymerization of tubulin subunits to form microtubule polymers. Many cell movements are mediated by microtubule action, including the beating of cilia and flagella, cytoplasmic transport of membrane vesicles, chromosome alignment during meiosis/mitosis, and nerve-cell axon migration. These movements result from competitive microtubule polymerization and depolymerization or through the actions of microtubule motor proteins (1).β3-tubulin (TUBB3) is one of six β-tubulin isoforms and is expressed highly during fetal and postnatal development (axon guidance and maturation) (2). Its expression levels decrease in the adult central nervous system (CNS) but remain high in the peripheral nervous system (PNS) (3). Microtubules enriched in β3-tubulin are more dynamic than those composed of other β-tubulin isoforms (4). Research studies have shown that mutations in the β3-tubulin gene TUBB3 cause ocular motility defects and other nervous system disorders. Furthermore, β3-tubulin is present in neoplastic but not in normal differentiated glial cells. Thus, β3-tubulin is a great neuronal marker (5,6).

  1. Westermann, S. and Weber, K. (2003) Nat Rev Mol Cell Biol 4, 938-47.
  2. Katsetos, C.D. et al. (2003) J Child Neurol 18, 851-66; discussion 867.
  3. Jiang, Y.Q. and Oblinger, M.M. (1992) J Cell Sci 103 ( Pt 3), 643-51.
  4. Panda, D. et al. (1994) Proc Natl Acad Sci U S A 91, 11358-62.
  5. Tischfield, M.A. et al. (2010) Cell 140, 74-87.
  6. Katsetos, C.D. et al. (2003) J Child Neurol 18, 851-66; discussion 867.

Application References

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Companion Products

For Research Use Only. Not For Use In Diagnostic Procedures.

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