Product Pathways - Tyrosine Kinase / Adaptors
Tyro3 (D38C6) Rabbit mAb #5585
|W IP||H M R Mk||Endogenous||110, 130||Rabbit IgG|
Reactivity Key: H=Human M=Mouse R=Rat Mk=Monkey
Species cross-reactivity is determined by western blot. Species enclosed in parentheses are predicted to react based on 100% sequence homology.
Specificity / Sensitivity
Tyro3 (D38C6) Rabbit mAb detects endogeneous levels of total Tyro3 protein.
Source / Purification
Monoclonal antibody is produced by immunizing animals with a synthetic peptide corresponding to residues surrounding Thr874 of human Tyro3 protein.
Tyro3 is a receptor tyrosine kinase belonging to the TAM subfamily (Tyro3, Axl and Mer). All three members have similar domain structure composed of an extracellular region with 2 Ig-like domains, followed by 2 FNII-like domains, a single transmembrane region, and a cytoplasmic tyrosine kinase domain (1). The natural ligand for Tyro3, as well as Axl and Mer, is Gas6 (growth arrest-specific gene 6) (1,2). Expression pattern and target knockout data indicate an important role of Tyro3 in apoptotic cell phagocytosis of dendritic cells and macrophages (3), NK cell differentiation (4), reproductive neuron survival and migration (5), osteoclast stimulation (6,7), as well as cortical and hippocampal neuron function (8). Both MAPK and PI3K pathways have been suggested as downstream targets of Tyro3 activation (7,8). Tyro3 has also been shown to be correlated to melanoma tumorgenesis, likely through its reglulatory role in the expression of ongogenic microphthalmia-associated transcription factor (MITF) (9).
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- Prieto, A.L. et al. (2007) Neuroscience 150, 319-34.
- Zhu, S. et al. (2009) Proc Natl Acad Sci U S A 106, 17025-30.
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