Cell Signaling Technology

Product Pathways - Tyrosine Kinase / Adaptors

Tyro3 (D38C6) Rabbit mAb #5585

Applications Reactivity Sensitivity MW (kDa) Isotype
W IP H M R Mk Endogenous 110, 130 Rabbit IgG

Applications Key:  W=Western Blotting  IP=Immunoprecipitation
Reactivity Key:  H=Human  M=Mouse  R=Rat  Mk=Monkey
Species cross-reactivity is determined by western blot. Species enclosed in parentheses are predicted to react based on 100% sequence homology.

Protocols

Specificity / Sensitivity

Tyro3 (D38C6) Rabbit mAb detects endogeneous levels of total Tyro3 protein.

Source / Purification

Monoclonal antibody is produced by immunizing animals with a synthetic peptide corresponding to residues surrounding Thr874 of human Tyro3 protein.

Western Blotting

Western Blotting

Western blot analysis of extracts from COS-7 cells, and tissues from human and mouse brain using Tyro3 (D38C6) Rabbit mAb.

Background

Tyro3 is a receptor tyrosine kinase belonging to the TAM subfamily (Tyro3, Axl and Mer). All three members have similar domain structure composed of an extracellular region with 2 Ig-like domains, followed by 2 FNII-like domains, a single transmembrane region, and a cytoplasmic tyrosine kinase domain (1). The natural ligand for Tyro3, as well as Axl and Mer, is Gas6 (growth arrest-specific gene 6) (1,2). Expression pattern and target knockout data indicate an important role of Tyro3 in apoptotic cell phagocytosis of dendritic cells and macrophages (3), NK cell differentiation (4), reproductive neuron survival and migration (5), osteoclast stimulation (6,7), as well as cortical and hippocampal neuron function (8). Both MAPK and PI3K pathways have been suggested as downstream targets of Tyro3 activation (7,8). Tyro3 has also been shown to be correlated to melanoma tumorgenesis, likely through its reglulatory role in the expression of ongogenic microphthalmia-associated transcription factor (MITF) (9).

  1. Binder, M.D. and Kilpatrick, T.J. (2009) Neurosignals 17, 277-87.
  2. Nagata, K. et al. (1996) J Biol Chem 271, 30022-7.
  3. Seitz, H.M. et al. (2007) J Immunol 178, 5635-42.
  4. Caraux, A. et al. (2006) Nat Immunol 7, 747-54.
  5. Pierce, A. et al. (2008) Mol Endocrinol 22, 2481-95.
  6. Nakamura, Y.S. et al. (1998) Stem Cells 16, 229-38.
  7. Katagiri, M. et al. (2001) J Biol Chem 276, 7376-82.
  8. Prieto, A.L. et al. (2007) Neuroscience 150, 319-34.
  9. Zhu, S. et al. (2009) Proc Natl Acad Sci U S A 106, 17025-30.

Application References

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