Cell Signaling Technology
XP Monoclonal Antibody

Product Pathways - Cytoskeletal Signaling

Rab11 (D4F5) XP® Rabbit mAb #5589

Applications Reactivity Sensitivity MW (kDa) Isotype
W IP IF-IC H M R Mk (Hm) (Pg) Endogenous 25 Rabbit IgG

Applications Key:  W=Western Blotting  IP=Immunoprecipitation  IF-IC=Immunofluorescence (Immunocytochemistry)
Reactivity Key:  H=Human  M=Mouse  R=Rat  Hm=Hamster  Mk=Monkey  Pg=Pig
Species cross-reactivity is determined by western blot. Species enclosed in parentheses are predicted to react based on 100% sequence homology.

Protocols

Specificity / Sensitivity

Rab11 (D4F5) XP® Rabbit mAb detects endogeneous levels of Rab11 protein, including isoforms Rab11a and Rab11b.

Source / Purification

Monoclonal antibody is produced by immunizing animals with a synthetic peptide corresponding to residues near the amino terminus of human Rab11 protein.

Western Blotting

Western Blotting

Western blot analysis of extracts from various cell lines using Rab11 (D4F5) XP® Rabbit mAb.

IF-IC

IF-IC

Confocal immunofluorescent analysis of A549 cells using Rab11 (D4F5) XP® Rabbit mAb (green). Actin filaments were labeled wtih DY-554 phalloidin (red). Blue pseudocolor = DRAQ5® #4084 (fluorescent DNA dye).

Background

Rab11a, Rab11b and Rab25 are members of the Rab11 family of small Ras-like GTPases. Rab11 (isoforms Rab11a and Rab11b) functions as a key regulator in the recycling of perinuclear, plasma membrane and Golgi compartment endosomes (1,2). Despite some overlap, distinct differences exist between Rab11a and Rab11b in both their cellular distribution and functional roles. Rab11a is ubiquitously expressed while Rab11b is found mainly in the heart and brain (3,4). Like other Rab proteins, Rab11 exerts its function via interactions with Rab11 family interacting proteins (FIPs). While there are three distinct classes of FIPs, all appear to share a conserved carboxy-terminal Rab-binding domain that allows Rab-FIP protein interaction. When bound together, these proteins are thought to regulate membrane-associated protein sorting (5,6).

  1. Ullrich, O. et al. (1996) J. Cell Biol. 135, 913-924.
  2. Chen, W. et al. (1998) Mol. Biol. Cell 9, 3241-3257.
  3. Lapierre, L.A. et al. (2003) Exp. Cell Res. 290, 322-331.
  4. Khvotchev, M.V. et al. (2003) J. Neurosci. 23, 10531-10539.
  5. Junutula, J.R. et al. (2004) J. Biol. Chem. 279, 33430-33437.
  6. Hales, C.M. et al. (2001) J. Biol. Chem. 276, 39067-39075.

Application References

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Companion Products

For Research Use Only. Not For Use In Diagnostic Procedures.

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