Product Pathways - Cytoskeletal Signaling
Troponin T (Cardiac) Antibody #5593
PhosphoSitePlus® protein, site, and accession data: TNNT2
| Applications | Reactivity | Sensitivity | MW (kDa) | Source |
|---|---|---|---|---|
| W | H R (Mk) | Endogenous | 40 | Rabbit |
Applications Key:
W=Western Blotting
Reactivity Key:
H=Human
R=Rat
Mk=Monkey
Species cross-reactivity is determined by western blot. Species enclosed in parentheses are predicted to react based on 100% sequence homology.
Protocols
- 5593:
- Western Blotting
Specificity / Sensitivity
Troponin T (Cardiac) Antibody detects endogenous levels of total cardiac Troponin T protein.
Source / Purification
Polyclonal antibodies are produced by immunizing animals with a synthetic peptide corresponding to a region surrounding Pro69 of human cardiac Troponin T protein. Antibodies are purified by protein A and peptide affinity chromatography.
Western Blotting
Western blot analysis of extracts from 293T cells, mock transfected (-) or transfected with a human cardiac Troponin T (isoform 1) construct (+), using Troponin T (Cardiac) Antibody.
Western Blotting
Western blot analysis of extracts from human and rat heart and human skeletal muscle using Troponin T (Cardiac) Antibody.
Background
Troponin, working in conjunction with tropomyosin, functions as a molecular switch, regulating muscle contraction in response to changes in the intracellular Ca2+ concentration. Troponin consists of three subunits: the Ca2+-binding subunit troponin C (TnC), the tropomyosin-binding subunit troponin T (TnT), and the inhibitory subunit troponin I (TnI) (1). In response to β-adrenergic stimulation of the heart, Ser23 and Ser24 of TnI (cardiac) are phosphorylated by PKA and PKC. This phosphorylation stimulates a conformational change of the regulatory domain of TnC, reduces the association between TnI and TnC, and decreases myofilament Ca2+ sensitivity by reducing the Ca2+ binding affinity of TnC (1-3).
The tropomyosin binding subunit of the troponin complex TnT exists as different isoforms in slow skeletal muscle (ssTnT/TNNT1), fast skeletal muscle (fsTnT/TNNT3) and in cardiac muscle (cTnT/TNNT2). Each of these may also contain multiple alternatively spliced variants. Assays for measuring serum concentrations of cTnT, as well as cTnI, have been reported for analyzing cardiac injury.
- Ward, D.G. et al. (2002) J. Biol. Chem. 277, 41795-41801.
- Noland, T.A. et al. (1995) J. Biol. Chem. 270, 25445-25454.
- Gaponenko, V. et al. (1999) J. Biol. Chem. 274, 16681-16684.
Application References
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Companion Products
- 4004 Phospho-Troponin I (Cardiac) (Ser23/24) Antibody
- 4002 Troponin I Antibody
- 7071 Phototope®-HRP Western Blot Detection System, Anti-rabbit IgG, HRP-linked Antibody
- 7074 Anti-rabbit IgG, HRP-linked Antibody
- 7720 Prestained Protein Marker, Broad Range (Premixed Format)
- 7727 Biotinylated Protein Ladder Detection Pack
- 7003 20X LumiGLO® Reagent and 20X Peroxide
For Research Use Only. Not For Use In Diagnostic Procedures.
