Cell Signaling Technology

Product Pathways - PI3K / Akt Signaling

SGK2 Antibody #5595

Applications Reactivity Sensitivity MW (kDa) Source
W IP H M R Endogenous 42 Rabbit

Applications Key:  W=Western Blotting  IP=Immunoprecipitation
Reactivity Key:  H=Human  M=Mouse  R=Rat
Species cross-reactivity is determined by western blot. Species enclosed in parentheses are predicted to react based on 100% sequence homology.

Protocols

Specificity / Sensitivity

SGK2 Antibody detects endogenous levels of total SGK2 protein

Source / Purification

Polyclonal antibodies are produced by immunizing animals with a synthetic peptide corresponding to residues surrounding Phe386 of human SGK2 protein. Antibodies are purified by protein A and peptide affinity chromatography.

Western Blotting

Western Blotting

Western blot analysis of extracts from human and mouse liver tissues using SGK2 Antibody.

Western Blotting

Western Blotting

Western blot analysis of extracts from COS-7 cells, untransfected (-) or transfected with human SGK2 (+), using SGK2 Antibody.

Background

Serum and glucocorticoid-inducible kinase (SGK) is a serine/threonine kinase closely related to Akt (1). SGK is rapidly induced in response to a variety of stimuli, including serum, glucocorticoid, follicle stimulating hormone, osmotic shock, and mineralocorticoids. SGK activation can be accomplished via HGF PI3K-dependent pathways and by integrin-mediated PI3K-independent pathways (2,3). Induction and activation of SGK has been implicated in activating the modulation of anti-apoptotic and cell cycle regulation (4-6). SGK also plays an important role in activating certain potassium, sodium, and chloride channels, suggesting its involvement in the regulation of processes such as cell survival, neuronal excitability, and renal sodium excretion (2). SGK is negatively regulated by ubiquitination and proteasome degradation (7).

  1. Webster, M.K. et al. (1993) Mol Cell Biol 13, 2031-40.
  2. Kobayashi, T. and Cohen, P. (1999) Biochem J 339 ( Pt 2), 319-28.
  3. Park, J. et al. (1999) EMBO J 18, 3024-33.
  4. Brunet, A. et al. (2001) Mol Cell Biol 21, 952-65.
  5. Mikosz, C.A. et al. (2001) J Biol Chem 276, 16649-54.
  6. Hayashi, M. et al. (2001) J Biol Chem 276, 8631-4.
  7. Brickley, D.R. et al. (2002) J Biol Chem 277, 43064-70.

Application References

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Companion Products

For Research Use Only. Not For Use In Diagnostic Procedures.

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