Product Pathways - PI3K / Akt Signaling
Phospho-SGK1 (Ser78) (D36D11) Rabbit mAb #5599
|5599S||100 µl (10 western blots)||---||In Stock||---|
|5599||carrier free and custom formulation / quantity||email request|
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Species cross-reactivity is determined by western blot.
Applications Key: W=Western Blotting, IP=Immunoprecipitation
Species predicted to react based on 100% sequence homology: Mouse, Rat, Monkey.
Specificity / Sensitivity
Phospho-SGK1 (Ser78) (D36D11) Rabbit mAb detects endogeneous levels of SGK1 protein only when phosphorylated at Ser78. This antibody does not detect isoforms SGK2 or SGK3.
Source / Purification
Monoclonal antibody is produced by immunizing animals with a synthetic phosphopeptide corresponding to residues surrounding Ser78 of human SGK1 protein.
Western blot analysis of extracts from COS cells transfected with SGK1 using Phospho-SGK1 (Ser78) (D36D11) Rabbit mAb, untreated or following antibody pre-incubation with either a site-specific phosphorylated peptide to block the signal or a site-specific non-phosphorylated peptide that cannot block the signal.
Western blot analysis of extracts from HeLa cells, untreated (-) or treated with H2O2 (4 mM for 15 min), using Phospho-SGK1 (Ser78) (D36D11) (upper) or SGK1 Antibody #3272 (lower).
Western blot analysis of extracts from COS cells, untransfected (-) or transfected with human SGK1 (+), using Phospho-SGK1 (Ser78) (D36D11) (upper) or SGK1 Antibody #3272 (lower).
Serum and glucocorticoid-inducible kinase (SGK) is a serine/threonine kinase closely related to Akt (1). SGK is rapidly induced in response to a variety of stimuli, including serum, glucocorticoid, follicle stimulating hormone, osmotic shock, and mineralocorticoids. SGK activation can be accomplished via HGF PI3K-dependent pathways and by integrin-mediated PI3K-independent pathways (2,3). Induction and activation of SGK has been implicated in activating the modulation of anti-apoptotic and cell cycle regulation (4-6). SGK also plays an important role in activating certain potassium, sodium, and chloride channels, suggesting its involvement in the regulation of processes such as cell survival, neuronal excitability, and renal sodium excretion (2). SGK is negatively regulated by ubiquitination and proteasome degradation (7).
The MAP kinase family member BMK1 interacts with and activates SGK by phosphorylation at serine 78 (6).
- Webster, M.K. et al. (1993) Mol Cell Biol 13, 2031-40.
- Kobayashi, T. and Cohen, P. (1999) Biochem J 339 ( Pt 2), 319-28.
- Park, J. et al. (1999) EMBO J 18, 3024-33.
- Brunet, A. et al. (2001) Mol Cell Biol 21, 952-65.
- Mikosz, C.A. et al. (2001) J Biol Chem 276, 16649-54.
- Hayashi, M. et al. (2001) J Biol Chem 276, 8631-4.
- Brickley, D.R. et al. (2002) J Biol Chem 277, 43064-70.
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For Research Use Only. Not For Use In Diagnostic Procedures.
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