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hnRNP A0 (D26A2) Rabbit mAb #5600

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PhosphoSitePlus^® protein, site, and accession data: hnRNP A0

Applications	Reactivity	Sensitivity	MW (kDa)	Isotype
W IP	H M R	Endogenous	32, 34	Rabbit IgG

Applications Key: W=Western Blotting IP=Immunoprecipitation
Reactivity Key: H=Human M=Mouse R=Rat
Species cross-reactivity is determined by western blot. Species enclosed in parentheses are predicted to react based on 100% sequence homology.

Protocols

5600:: Immunoprecipitation, Western Blotting

Specificity / Sensitivity

hnRNP A0 (D26A2) Rabbit mAb recognizes endogenous levels of total hnRNP A0 protein. This antibody does not cross-react with other hnRNP family members.

Source / Purification

Monoclonal antibody is produced by immunizing animals with a synthetic peptide corresponding to residues surrounding Ala152 of human hnRNP A0 protein.

Western Blotting

Western blot analysis of extracts from various cell lines using hnRNP A0 (D26A2) Rabbit mAb.

Background

Heterogeneous nuclear ribonucleoprotein A0 (hnRNP A0) is a member of the hnRNP A/B family of related RNA binding proteins that bind pre-mRNA and are involved in the processing, metabolism, and transport of nuclear pre-mRNA transcripts (1). The A/B subfamily of hnRNP includes A1, A2/B1, A3, and A0. hnRNP A0 is phosphorylated at Ser84 by MAPKAPK-2 in response to LPS treatment in mouse macrophage cells, which might play a key role in stimulating translation of the TNF-α message (2).

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For Research Use Only. Not For Use In Diagnostic Procedures.