Cell Signaling Technology
XP Monoclonal Antibody

Product Pathways - Adhesion

βIG-H3 (D31B8) XP® Rabbit mAb #5601

Applications Reactivity Sensitivity MW (kDa) Isotype
W IP IF-IC H Endogenous 70 Rabbit IgG

Applications Key:  W=Western Blotting  IP=Immunoprecipitation  IF-IC=Immunofluorescence (Immunocytochemistry)
Reactivity Key:  H=Human
Species cross-reactivity is determined by western blot. Species enclosed in parentheses are predicted to react based on 100% sequence homology.

Protocols

Specificity / Sensitivity

βIG-H3 (D31B8) XP® Rabbit mAb detects endogenous levels of total βIG-H3 protein.

Source / Purification

Monoclonal antibody is produced by immunizing animals with a synthetic peptide corresponding to residues near the carboxy terminus of human βIG-H3 protein.

Western Blotting

Western Blotting

Western blot analysis of extracts from A549 cells treated with hTGFβ-3 #8425 (100 ng/ml for 12 hours) and OVCAR8 cells using βIG-H3 (D31B8) XP® Rabbit mAb.

IF-IC

IF-IC

Confocal immunofluorescent analysis of A549 cells, untreated (left) or treated with hTGF-β-1 #8915 (100 ng/ml for 24 hours, right), using βIG-H3 (D31B8) XP® Rabbit mAb (green). Actin filaments were labeled with DY-554 phalloidin. Blue pseudocolor = DRAQ5® #4084 (fluorescent DNA dye).

Background

βIG-H3 (TGFBI/RGD-CAP/Kerato-epithelin) is a 683-amino acid secretory protein induced by TGF-β that plays a role in cell adhesion, differentiation, and apoptosis (1-4). βIG-H3 contains an internal cysteine-rich EMI domain followed by four fasciclin-1 domains and a carboxy terminal RGD domain (1,2). It contributes to cell adhesion through interactions with integrins as well as a number of extracellular matrix (ECM) proteins including collagen, fibronectin, and laminin (5-7). ECM βIG-H3 is found in a wide variety of tissues (8-12). Mutations in the βIG-H3 gene as well as elevated protein levels are most notably associated with corneal dystrophies (13).

  1. Skonier, J. et al. (1992) DNA Cell Biol 11, 511-22.
  2. Skonier, J. et al. (1994) DNA Cell Biol 13, 571-84.
  3. Hashimoto, K. et al. (1997) Biochim Biophys Acta 1355, 303-14.
  4. Kim, J.E. et al. (2003) Oncogene 22, 2045-53.
  5. Kim, J.E. et al. (2002) Invest Ophthalmol Vis Sci 43, 656-61.
  6. Billings, P.C. et al. (2002) J Biol Chem 277, 28003-9.
  7. Hanssen, E. et al. (2003) J Biol Chem 278, 24334-41.
  8. Gibson, M.A. et al. (1997) J Histochem Cytochem 45, 1683-96.
  9. Billings, P.C. et al. (2000) Am J Respir Cell Mol Biol 22, 352-9.
  10. Gilbert, R.E. et al. (1998) Kidney Int 54, 1052-62.
  11. Rawe, I.M. et al. (1997) Invest Ophthalmol Vis Sci 38, 893-900.
  12. LeBaron, R.G. et al. (1995) J Invest Dermatol 104, 844-9.
  13. Munier, F.L. et al. (1997) Nat Genet 15, 247-51.

Application References

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Companion Products

For Research Use Only. Not For Use In Diagnostic Procedures.

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