Product Pathways - Neuroscience
BACE (D10E5) Rabbit mAb #5606
|5606S||100 µl (10 western blots)||---||In Stock||---|
|5606P||40 µl (4 western blots)||---||In Stock||---|
|5606||carrier free and custom formulation / quantity||email request|
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|W||1:1000||Human, Mouse, Rat||Endogenous||70||Rabbit IgG|
Species cross-reactivity is determined by western blot.
Applications Key: W=Western Blotting, IP=Immunoprecipitation, IF-F=Immunofluorescence (Frozen), IF-IC=Immunofluorescence (Immunocytochemistry)
Specificity / Sensitivity
BACE (D10E5) Rabbit mAb detects endogenous levels of total BACE protein.
Source / Purification
Monoclonal antibody is produced by immunizing animals with a synthetic peptide corresponding to residues surrounding His490 of human BACE protein.
Western blot analysis of extracts from HeLa cells, untransfected and BACE transfected, and rat brain extracts using BACE (D10E5) Rabbit mAb.
Confocal immunfluorescent analysis of H4 expressing BACE1 (left), or H4 wild-type, (right) cells using BACE1 (D10E5) Rabbit mAb (green). Actin filaments were labeled with DyLight® 554 Phalloidin #13054 (red). Blue pseudocolor = DRAQ5® #4084 (fluorescent DNA dye). (H4 wild type and H4 cells expressing BACE1 were kindly provided by Dr. Giuseppina Tesco, Tufts University, Boston, MA).
Confocal immunofluorescent analysis of mouse hippocampus using BACE1 (D10E5) Rabbit mAb (green). Blue pseudocolor = DRAQ5® #4084 (fluorescent DNA dye).
β-secretase (BACE) is an aspartic acid proteinase that catalyses the initial step in APP processing by cleaving and releasing a soluble, extracellular APP-β (sAPPβ) ectodomain and generating a membrane-bound, carboxy-terminal fragment consisting of 99 amino acids (CTF99). Additional processing of CTF99 by γ-secretase generates the amyloid β-peptide (Aβ) that forms aggregates in the brains of Alzheimer disease (AD) patients. BACE is an attractive target for inhibitors in AD therapy since it catalyses the first and rate limiting step in amyloidogenic APP processing (1). Pro-BACE-1 is synthesized in the ER before it is transported to the trans-Golgi network to undergo maturation (2). BACE-1 functions as a dimer and is phosphorylated at Ser498 by casein kinase 1, which is necessary for recycling back to the membrane from the early endosome (3,4).
- Giusti-Rodríguez, P. et al. (2011) J Neurosci 31, 15751-6. Applications: Western Blotting.
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For Research Use Only. Not For Use In Diagnostic Procedures.
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