Product Pathways - Neuroscience
BACE (D10E5) Rabbit mAb #5606
PhosphoSitePlus® protein, site, and accession data: BACE
| Applications | Reactivity | Sensitivity | MW (kDa) | Isotype |
|---|---|---|---|---|
| W IP | H M R | Endogenous | 70 | Rabbit IgG |
Applications Key:
W=Western Blotting
IP=Immunoprecipitation
Reactivity Key:
H=Human
M=Mouse
R=Rat
Species cross-reactivity is determined by western blot. Species enclosed in parentheses are predicted to react based on 100% sequence homology.
Protocols
Specificity / Sensitivity
BACE (D10E5) Rabbit mAb detects endogenous levels of total BACE protein.
Source / Purification
Monoclonal antibody is produced by immunizing animals with a synthetic peptide corresponding to residues surrounding His490 of human BACE protein.
Western Blotting
Western blot analysis of extracts from HeLa cells, untransfected and BACE transfected, and rat brain extracts using BACE (D10E5) Rabbit mAb.
Background
β-secretase (BACE) is an aspartic acid proteinase that catalyses the initial step in APP processing by cleaving and releasing a soluble, extracellular APP-β (sAPPβ) ectodomain and generating a membrane-bound, carboxy-terminal fragment consisting of 99 amino acids (CTF99). Additional processing of CTF99 by γ-secretase generates the amyloid β-peptide (Aβ) that forms aggregates in the brains of Alzheimer disease (AD) patients. BACE is an attractive target for inhibitors in AD therapy since it catalyses the first and rate limiting step in amyloidogenic APP processing (1). Pro-BACE-1 is synthesized in the ER before it is transported to the trans-Golgi network to undergo maturation (2). BACE-1 functions as a dimer and is phosphorylated at Ser498 by casein kinase 1, which is necessary for recycling back to the membrane from the early endosome (3,4).
- Hunt, C.E. and Turner, A.J. (2009) FEBS J 276, 1845-59.
- Benjannet, S. et al. (2001) J Biol Chem 276, 10879-87.
- Walter, J. et al. (2001) J Biol Chem 276, 14634-41.
- He, X. et al. (2005) J Biol Chem 280, 11696-703.
Application References
- Giusti-RodrÃguez, P. et al. (2011) J Neurosci 31, 15751-6. Applications: Western Blotting
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For Research Use Only. Not For Use In Diagnostic Procedures.
