Product Pathways - Ca / cAMP / Lipid Signaling
MARCKS (D88D11) XP® Rabbit mAb #5607
PhosphoSitePlus® protein, site, and accession data: MARCKS
| Applications | Reactivity | Sensitivity | MW (kDa) | Isotype |
|---|---|---|---|---|
| W IHC-P IF-IC F | H Mk | Endogenous | 75 | Rabbit IgG |
Applications Key:
W=Western Blotting
IHC-P=Immunohistochemistry (Paraffin)
IF-IC=Immunofluorescence (Immunocytochemistry)
F=Flow Cytometry
Reactivity Key:
H=Human
Mk=Monkey
Species cross-reactivity is determined by western blot. Species enclosed in parentheses are predicted to react based on 100% sequence homology.
Protocols
Specificity / Sensitivity
MARCKS (D88D11) XP® Rabbit mAb recognizes endogenous levels of total MARCKS protein.
Source / Purification
Monoclonal antibody is produced by immunizing animals with a synthetic peptide corresponding to residues surrounding Ala245 of human MARCKS protein.
Western Blotting
Western blot analysis of cell extracts from various cell lines using MARCKS (D88D11) XP® Rabbit mAb.
IHC-P (paraffin)
Immunohistochemical analys of paraffin-embedded human breast carcinoma using MARCKS (D88D11) XP® Rabbit mAb.
IHC-P (paraffin)
Immunohistochemical analys of paraffin-embedded human breast carcinoma using MARCKS (D88D11) XP® Rabbit mAb.
IHC-P (paraffin)
Immunohistochemical analysis of paraffin-embedded 293 (left) or K562 (right) cell pellets using MARCKS (D88D11) XP® Rabbit mAb.
Flow Cytometry
Flow cytometric analysis of Jurkat (blue) and HeLa (green) cells using MARCKS (D88D11) XP® Rabbit mAb.
IF-IC
Confocal immunofluorescent analysis of HeLa (left), MCF-7 (middle), and Jurkat (right) cells using MARCKS (D88D11) XP® Rabbit mAb (green). Actin filaments were labeled with DY-554 phalloidin (red). Blue pseudocolor = DRAQ5® #4084 (fluorescent DNA dye).
Background
Myristoylated Alanine-Rich C-Kinase Substrate (MARCKS) is a major PKC substrate expressed in many cell types. MARCKS has been implicated in cell motility, cell adhesion, phagocytosis, membrane traffic, and mitogenesis (1). PKC phosphorylates Ser159, 163, 167, and 170 of MARCKS in response to growth factors and oxidative stress. Phosphorylation at these sites regulates the calcium/calmodulin binding and filamentous (F)-actin cross-linking activities of MARCKS (2-4). Phosphorylation by PKC also results in translocation of MARCKS from the plasma membrane to the cytoplasm (5).
- Ramsden, J.J. (2000) Int J Biochem Cell Biol 32, 475-9.
- Heemskerk, F.M. et al. (1993) Biochem Biophys Res Commun 190, 236-41.
- Graff, J.M. et al. (1989) J Biol Chem 264, 21818-23.
- Hartwig, J.H. et al. (1992) Nature 356, 618-22.
- Thelen, M. et al. (1991) Nature 351, 320-2.
Application References
Have you published research involving the use of our products? If so we'd love to hear about it. Please let us know!
Companion Products
- 2741 Phospho-MARCKS (Ser152/156) Antibody
- 9921 Phospho-PKC Antibody Sampler Kit
- 7071 Phototope®-HRP Western Blot Detection System, Anti-rabbit IgG, HRP-linked Antibody
- 7074 Anti-rabbit IgG, HRP-linked Antibody
- 7720 Prestained Protein Marker, Broad Range (Premixed Format)
- 7727 Biotinylated Protein Ladder Detection Pack
- 7003 20X LumiGLO® Reagent and 20X Peroxide
- 8112 SignalStain® Antibody Diluent
- 8114 SignalStain® Boost IHC Detection Reagent (HRP, Rabbit)
- 8059 SignalStain® DAB Substrate Kit
For Research Use Only. Not For Use In Diagnostic Procedures.
