Cell Signaling Technology

Product Pathways - Cytoskeletal Signaling

ARP2 (D85D5) Rabbit mAb #5614

Applications Reactivity Sensitivity MW (kDa) Isotype
W H M R Mk B Endogenous 44 Rabbit IgG

Applications Key:  W=Western Blotting
Reactivity Key:  H=Human  M=Mouse  R=Rat  Mk=Monkey  B=Bovine
Species cross-reactivity is determined by western blot. Species enclosed in parentheses are predicted to react based on 100% sequence homology.

Protocols

Specificity / Sensitivity

ARP2 (D85D5) Rabbit mAb recognizes endogenous levels of total ARP2 protein.

Source / Purification

Monoclonal antibody is produced by immunizing animals with a synthetic peptide corresponding to residues near the carboxy terminus of human ARP2 protein.

Western Blotting

Western Blotting

Western blot analysis of extracts from various cell lines using ARP2 (D85D5) Rabbit mAb.

Background

Actin nucleation, the formation of new actin filaments from existing filaments, affects actin filament structure during cell motility, division, and intracellular trafficking. An important actin nucleation protein complex is the highly conserved ARP2/3 complex, consisting of ARP2, ARP3, and ARPC1-5. The ARP2/3 complex promotes branching of an existing actin filament and formation of a daughter filament following activation by nucleation-promoting factors, such as WASP/WAVE or cortactin (1). The formation of podosomes, small cellular projections that degrade the extracellular matrix, is enhanced by ARP2/3 complex action. ARP2/3 competes with caldesmon, an actin binding protein shown to negatively affect podosome formation (2). Along with N-WASP, the ARP2/3 complex regulates nuclear actin filament nucleation and controls actin polymerization during transcription (3).

  1. Goley, E.D. and Welch, M.D. (2006) Nat. Rev. Mol. Cell Biol. 7, 713-726.
  2. Morita, T. et al. (2007) J. Biol. Chem. 282, 8454-8463.
  3. Yoo, Y. et al. (2007) J. Biol. Chem. 282, 7616-7623.

Application References

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For Research Use Only. Not For Use In Diagnostic Procedures.

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