Product Pathways - Cytoskeletal Signaling
Phospho-ARHGAP42 (Tyr376) (D45E9) Rabbit mAb #5617
|W IP||H M Mk (R)||Endogenous||125||Rabbit IgG|
Reactivity Key: H=Human M=Mouse R=Rat Mk=Monkey
Species cross-reactivity is determined by western blot. Species enclosed in parentheses are predicted to react based on 100% sequence homology.
Specificity / Sensitivity
Phospho-ARHGAP42 (Tyr376) (D45E9) Rabbit mAb detects endogenous levels of ARHGAP42 only when phosphorylated at Tyr376.
Source / Purification
Monoclonal antibody is produced by immunizing animals with a synthetic phosphopeptide corresponding to residues surrounding Tyr376 of human ARHGAP42 protein.
Western blot analysis of extracts from COS-7 cells using Phospho-ARHGAP42 (Tyr376) (D45E9) Rabbit mAb. The phospho-specificity of the antibody was verified by preincubating the antibody with no peptide, phosphopeptide, or the corresponding non-phosphopeptide prior to incubating the membrane.
ARHGAP family members activate Rho GTPase proteins (1). Mutations of ARHGAP family genes lead to carcinogenesis through the dysregulation of Rho/Rac/Cdc42-like GTPases (2). Little is known about the biological function of ARHGAP42, a member of the ARHGAP family. ARHGAP42 contains a RhoGAP domain in the middle of its sequence, an SH3 binding domain at the C-terminal end of the protein sequence, as well as a BAR domain and a PH domain in the N-terminal region of the sequence, which indicate that the protein may function as GTPase activator and cytoskeletal adaptor.
- Katoh, M. and Katoh, M. (2004) Int J Mol Med 14, 333-8.
- Katoh, M. and Katoh, M. (2004) Int J Oncol 25, 1201-6.
Have you published research involving the use of our products? If so we'd love to hear about it. Please let us know!
- 7071 Phototope®-HRP Western Blot Detection System, Anti-rabbit IgG, HRP-linked Antibody
- 7074 Anti-rabbit IgG, HRP-linked Antibody
- 7727 Biotinylated Protein Ladder Detection Pack
- 7720 Prestained Protein Marker, Broad Range (Premixed Format)
- 7003 20X LumiGLO® Reagent and 20X Peroxide
For Research Use Only. Not For Use In Diagnostic Procedures.