Cell Signaling Technology

Product Pathways - Lymphocyte Signaling

CD44 (8E2) Mouse mAb #5640

Applications Reactivity Sensitivity MW (kDa) Isotype
W IP IF-IC F H M R Endogenous 80 Mouse IgG1

Applications Key:  W=Western Blotting  IP=Immunoprecipitation  IF-IC=Immunofluorescence (Immunocytochemistry)  F=Flow Cytometry
Reactivity Key:  H=Human  M=Mouse  R=Rat
Species cross-reactivity is determined by western blot. Species enclosed in parentheses are predicted to react based on 100% sequence homology.

Protocols

Specificity / Sensitivity

CD44 (8E2) Mouse mAb detects endogenous levels of total CD44 protein.

Source / Purification

Monoclonal antibody is produced by immunizing BALB/c mice with purified recombinant CD44 protein.

Western Blotting

Western Blotting

Western blot analysis of extracts from HeLa, C6 and HUVEC cells using CD44 (8E2) Mouse mAb.

Flow Cytometry

Flow Cytometry

Flow cytometric analysis of Hela cells using CD44 (8E2) Mouse mAb (blue) compared to a nonspecific negative control antibody (red).

IF-IC

IF-IC

Projected confocal z-stack of HeLa cells using CD44 (8E2) Mouse mAb (green). Actin filaments have been labeled with DY-554 phalloidin (red). Blue pseudocolor = DRAQ5® #4084 (fluorescent DNA dye).


Background

CD44 is a type I transmembrane glycoprotein that mediates cell-cell and cell-matrix interaction through its affinity for hyaluronic acid (HA) and possibly through other parts of the extracellular matrix (ECM). CD44 is highly polymorphic, possesses a number of alternative splice variants and undergoes extensive post-translational modifications (1,2). Increased surface levels of CD44 are characteristic of T cell activation, and expression of the protein is upregulated during the inflammatory response. Research studies have shown that interactions between CD44 and HER2 are linked to an increase in ovarian carcinoma cell growth (1-3). CD44 interacts with ezrin, radixin and moesin (ERM), linking the actin cytoskeleton to the plasma membrane and the ECM (4-6). CD44 is constitutively phosphorylated at Ser325 in resting cells. Activation of PKC results in phosphorylation of Ser291, dephosphorylation of Ser325, disassociation of ezrin from CD44, and directional motility (4).

  1. Goodison, S. et al. (1999) Mol. Pathol. 52, 189-196.
  2. Cichy, J. and Puré, E. (2003) J. Cell Biol. 161, 839-843.
  3. Bourguignon, L.Y. et al. (1997) J. Biol. Chem. 272, 27913-27918.
  4. Legg, J.W. et al. (2002) Nat. Cell Biol. 4, 399-407.
  5. Yonemura, S. et al. (1998) J. Cell Biol. 140, 885-895.
  6. Tsukita, S. et al. (1994) J. Cell Biol. 126, 391-401.

Application References

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Companion Products

For Research Use Only. Not For Use In Diagnostic Procedures.

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