Cell Signaling Technology

Product Pathways - Protein Stability

Ubc12 (D13D7) Rabbit mAb #5641

Applications Reactivity Sensitivity MW (kDa) Isotype
W IP H M R Mk Endogenous 21 Rabbit IgG

Applications Key:  W=Western Blotting  IP=Immunoprecipitation
Reactivity Key:  H=Human  M=Mouse  R=Rat  Mk=Monkey
Species cross-reactivity is determined by western blot. Species enclosed in parentheses are predicted to react based on 100% sequence homology.

Protocols

Specificity / Sensitivity

Ubc12 (D13D7) Rabbit mAb detects endogeneous levels of total Ubc12 protein.

Source / Purification

Monoclonal antibody is produced by immunizing animals with a synthetic peptide corresponding to residues surrounding Lys36 of human Ubc12 protein.

Western Blotting

Western Blotting

Western blot analysis of extracts from various cell lines using Ubc12 (D13D7) Rabbit mAb.

Background

Similar to ubiquitin, Nedd8 signals to target proteins through an enzymatic cascade composed of Nedd8-specific E1 (activating)- and E2 (conjugating)-enzymes (1,2). The E2 ligase specific for Nedd8 is Ubc12 (3-5). Ubc12 forms a heterodimeric conjugate with NEDD8 in order to catalyze the transfer of NEDD8 from E1 to lysine side chains of target proteins (1,2). Well known targets of NEDD8 belong to the cullin family of E3 ligases. Neddylation of cullin activates the related ubiquitin E3 complex by promoting its interaction with ubiquitin-E2 ligase and catalyzing downstream target protein ubiquitination (6-7). Neddylation of the cullins on SCFROC1, SCFβTrCP, and SCFSKP2 has been shown to be required for controlling important signaling targets such as IκB, NF-κB, and p27 Kip (8-10), thereby regulating cell cycle progression, signaling cascades, and developmental programming processes (11).

  1. Huang, D.T. et al. (2007) Nature 445, 394-8.
  2. Huang, D.T. et al. (2005) Mol Cell 17, 341-50.
  3. Liakopoulos, D. et al. (1998) EMBO J 17, 2208-14.
  4. Gong, L. and Yeh, E.T. (1999) J Biol Chem 274, 12036-42.
  5. Wada, H. et al. (2000) J Biol Chem 275, 17008-15.
  6. Sakata, E. et al. (2007) Nat Struct Mol Biol 14, 167-8.
  7. Kawakami, T. et al. (2001) EMBO J 20, 4003-12.
  8. Podust, V.N. et al. (2000) Proc Natl Acad Sci USA 97, 4579-84.
  9. Wu, K. et al. (2002) J Biol Chem 277, 516-27.
  10. Amir, R.E. et al. (2002) J Biol Chem 277, 23253-9.
  11. Herrmann, J. et al. (2007) Circ Res 100, 1276-91.

Application References

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