Product Pathways - Neuroscience
Presenilin 1 (D39D1) Rabbit mAb #5643
PhosphoSitePlus® protein, site, and accession data: PSEN1
| Applications | Reactivity | Sensitivity | MW (kDa) | Isotype |
|---|---|---|---|---|
| W IP | H M R Mk | Endogenous | 22 (CTF) | Rabbit IgG |
Applications Key:
W=Western Blotting
IP=Immunoprecipitation
Reactivity Key:
H=Human
M=Mouse
R=Rat
Mk=Monkey
Species cross-reactivity is determined by western blot. Species enclosed in parentheses are predicted to react based on 100% sequence homology.
Protocols
Specificity / Sensitivity
Presenilin 1 (D39D1) Rabbit mAb recognizes endogenous levels of total Presenilin 1 protein. The antibody may detect a nonspecific band at 60 kDa.
Source / Purification
Monoclonal antibody is produced by immunizing animals with recombinant protein specific to the carboxy terminus of human Presenilin 1 protein.
Western Blotting
Western blot analysis of extracts from HeLa, wild-type MEF, and Presenilin 1(-/-) MEF cells using Presenilin 1 (D39D1) Rabbit mAb (upper) and α/β-Tubulin Antibody #2148 (lower). (Wild-type MEF and Presenilin 1(-/-) MEF cells were kindly provided by Dr. Bart De Strooper, K.U.Leuven, Belgium).
Background
Presenilin 1 and presenilin 2 are transmembrane proteins belonging to the presenilin family. Mutation of presenilin genes has been linked to early onset of Alzheimer disease, probably due to presenilin's associated γ-secretase activity for amyloid-β protein processing (1,2). Endogenous presenilin mainly exists in a heterodimeric complex formed from the endoproteolytically processed amino-terminal (34 kDa) and carboxy-terminal (~20, 22, 23 kDa) fragments (CTF) (2,3).
- Haass, C. and DeStrooper, B. (1999) Science 286, 916-919.
- Kimberly, W.T. et al. (2000) J. Biol. Chem. 275, 3173-3178.
- Kim, T.W. et al. (1997) J. Biol. Chem. 272, 11006-11010.
Application References
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For Research Use Only. Not For Use In Diagnostic Procedures.
