Cell Signaling Technology
XP Monoclonal Antibody

Product Pathways - Chromatin Regulation / Epigenetics

MTA1 (D40D1) XP® Rabbit mAb #5647

Applications Reactivity Sensitivity MW (kDa) Isotype
W IHC-P H M R Mk Endogenous 78-82 Rabbit IgG

Applications Key:  W=Western Blotting  IHC-P=Immunohistochemistry (Paraffin)
Reactivity Key:  H=Human  M=Mouse  R=Rat  Mk=Monkey
Species cross-reactivity is determined by western blot. Species enclosed in parentheses are predicted to react based on 100% sequence homology.

Protocols

Specificity / Sensitivity

MTA1 (D40D1) XP® Rabbit mAb recognizes endogenous levels of total MTA1 protein.

Source / Purification

Monoclonal antibody is produced by immunizing animals with a synthetic peptide corresponding to residues near the carboxy terminus of human MTA1 protein.

Western Blotting

Western Blotting

Western blot analysis of extracts from MCF7 and A-204 cells using MTA1 (D40D1) XP® Rabbit mAb.

IHC-P (paraffin)

IHC-P (paraffin)

Immunohistochemical analysis of paraffin-embedded mouse 4T1A mouse syngeneic tumor using MTA1 (D40D1) XP® Rabbit mAb.

IHC-P (paraffin)

IHC-P (paraffin)

Immunohistochemical analysis of paraffin-embedded human lung carcinoma using MTA1 (D40D1) XP® Rabbit mAb in the presence of control peptide (left) or antigen-specific peptide (right).


Background

MTA1 (metastasis associated gene 1) was identified in a differential screening of a cDNA library of metastatic and nonmetastatic adenocarcinoma cell lines (1), and was subsequently found to be an integral member of the nucleosome remodeling and deacetylation (NuRD) complex (2,3). MTA1 expression is upregulated under hypoxic conditions and found to enhance angiogenesis through stabilization of HIF-1α (4,5). MTA1 is overexpressed in a wide range of human cancers, and its expression is associated with malignancy and tumor progression (6). MTA1 is an essential downstream effector of c-Myc transformation (7). Recently, MTA1 was demonstrated to play a role in DNA damage response (8,9).

  1. Toh, Y. et al. (1994) J Biol Chem 269, 22958-63.
  2. Xue, Y. et al. (1998) Mol Cell 2, 851-61.
  3. Zhang, Y. et al. (1998) Cell 95, 279-89.
  4. Yoo, Y.G. et al. (2006) EMBO J 25, 1231-41.
  5. Moon, H.E. et al. (2006) Oncol Rep 16, 929-35.
  6. Toh, Y. and Nicolson, G.L. (2009) Clin Exp Metastasis 26, 215-27.
  7. Zhang, X.Y. et al. (2005) Proc Natl Acad Sci U S A 102, 13968-73.
  8. Li, D.Q. et al. (2009) J Biol Chem 284, 34545-52.
  9. Li, D.Q. et al. (2010) J Biol Chem 285, 10044-52.

Application References

Have you published research involving the use of our products? If so we'd love to hear about it. Please let us know!

Companion Products

Cell Signaling Technology® is a registered trademark of Cell Signaling Technology, Inc.

For Research Use Only. Not For Use In Diagnostic Procedures.

Products