Cell Signaling Technology

Product Pathways - Development

WIF1 (D50F9) Rabbit mAb #5652

Applications Reactivity Sensitivity MW (kDa) Isotype
W H Endogenous 42 Rabbit IgG

Applications Key:  W=Western Blotting
Reactivity Key:  H=Human
Species cross-reactivity is determined by western blot. Species enclosed in parentheses are predicted to react based on 100% sequence homology.

Protocols

Specificity / Sensitivity

WIF1 (D50F9) Rabbit mAb detects endogenous levels of total WIF1 protein.

Source / Purification

Monoclonal antibody is produced by immunizing animals with a synthetic peptide corresponding to residues surrounding Pro168 of human WIF1 protein.

Western Blotting

Western Blotting

Western blot analysis of extracts from COS-7 cells, transfected with a human WIF1 construct (+) or mock-transfected (-), and normal human lung using WIF1 (D50F9) Rabbit mAb.

Background

WIF1 (Wnt inhibitory factor 1) is a secreted protein that binds to Wnt proteins and inhibits their activity (1). It contains an N-terminal WIF domain and five EGF-like repeats (2). The WIF1 ortholog in Drosophila, Shifted, is required for Hedgehog stability and diffusion (3,4). It has been reported that WIF1 expression is downregulated in many types of cancers (5-8).

  1. Hsieh, J.C. et al. (1999) Nature 398, 431-6.
  2. Liepinsh, E. et al. (2006) J Mol Biol 357, 942-50.
  3. Gorfinkiel, N. et al. (2005) Dev Cell 8, 241-53.
  4. Glise, B. et al. (2005) Dev Cell 8, 255-66.
  5. Wissmann, C. et al. (2003) J Pathol 201, 204-12.
  6. Taniguchi, H. et al. (2005) Oncogene 24, 7946-52.
  7. Mazieres, J. et al. (2004) Cancer Res 64, 4717-20.
  8. Mazieres, J. et al. (2005) Cancer Lett 222, 1-10.

Application References

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