Cell Signaling Technology

Product Pathways - Ca / cAMP / Lipid Signaling

PKD3/PKCν (D57E6) Rabbit mAb #5655

Applications Reactivity Sensitivity MW (kDa) Isotype
W IP H M R Mk B Endogenous 110 Rabbit IgG

Applications Key:  W=Western Blotting  IP=Immunoprecipitation
Reactivity Key:  H=Human  M=Mouse  R=Rat  Mk=Monkey  B=Bovine
Species cross-reactivity is determined by western blot. Species enclosed in parentheses are predicted to react based on 100% sequence homology.

Protocols

Specificity / Sensitivity

PKD3/PKCν (D57E6) Rabbit mAb recognizes endogenous levels of total PKD3/PKCν protein.

Source / Purification

Monoclonal antibody is produced by immunizing animals with a synthetic peptide corresponding to residues surrounding Ala880 of human PKD3/PKCν protein.

Western Blotting

Western Blotting

Western blot analysis of extracts from various cell lines using PKD3/PKCν (D57E6) Rabbit mAb.

Background

PKCν, also known as PKD3, is a member of the protein kinase C (PKC) family of serine/threonine kinases that play critical roles in the regulation of cellular differentiation and proliferation. PKCν is composed of 890 amino acid residues and has 77.3% similarity to human PKCμ (PKCμ) and 77. 4% similarity to mouse PKD (the mouse homolog of PKCμ) (1). The PKCν mRNA is ubiquitously expressed in various tissues. PKCν has two putative diacylglycerol binding C1 domains, suggesting that it may participate in a novel diacylglycerol-mediated signaling pathway (2). PKCν is translocated to the plasma membrane and activated by the diacylglycerol mimic phorbol 12-myristate 13-acetate. PKCν is an important physiologic target of the B-cell receptor (BCR) and exhibits robust activation after BCR engagement (2). GPCR agonists induce a rapid activation of PKCν by a protein kinase C (PKC)-dependent pathway that leads to the phosphorylation of the activation loop of PKCν. PKCν is present both in the nucleus and cytoplasm and this distribution of PKCν results from its continuous shuttling between both compartments by a mechanism that requires a nuclear import receptor and a competent CRM1-nuclear export pathway (3). Cell stimulation with the GPCR agonist neurotensin induces a rapid and reversible plasma membrane translocation of PKCν that is PKC-dependent.

  1. Hayashi, A. et al. (1999) Biochim. Biophys. Acta. 1450, 99-106.
  2. Matthews, S.A. et al. (2003) J. Biol. Chem. 278, 9086-91.
  3. Rey, O. et al. (2003) J. Biol. Chem. 278, 23773-85.

Application References

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Companion Products

For Research Use Only. Not For Use In Diagnostic Procedures.

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