Cell Signaling Technology

Product Pathways - Neuroscience

RAIG1 Antibody #5657

Applications Reactivity Sensitivity MW (kDa) Source
W IF-IC H Endogenous 40-50 Rabbit

Applications Key:  W=Western Blotting  IF-IC=Immunofluorescence (Immunocytochemistry)
Reactivity Key:  H=Human
Species cross-reactivity is determined by western blot. Species enclosed in parentheses are predicted to react based on 100% sequence homology.

Protocols

Specificity / Sensitivity

RAIG1 Antibody recognizes endogenous levels of total RAIG1 protein.

Source / Purification

Polyclonal antibodies are produced by immunizing animals with a synthetic peptide corresponding to residues surrounding Val295 of human RAIG1 protein. Antibodies are purified by protein A and peptide affinity chromatography.

Western Blotting

Western Blotting

Western blot analysis of extracts from untransfected or human RAIG1-transfected 293T cells and untransfected MKN-45 cells using RAIG1 Antibody.

IF-IC

IF-IC

Confocal immunofluorescent analysis of MKN-45 (left) and 293 (right) cells using RAIG1 Antibody (green). Actin filaments have been labeled with DY-554 phalloidin. Blue pseudocolor = DRAQ5® #4084 (fluorescent DNA dye).

Background

Retinoic Acid-Induced Gene 1 (RAIG1), also called RAI3 and GPRC5A, belongs to the family of G protein-coupled receptors (GPCRs). GPCRs are the largest and most abundant receptor family in mammals, composed of more than one thousand members (1). Retinoic Acids (RA) are a group of pleiotropic signaling molecules that regulate a broad range of physiologic and developmental processes. It is thought that RAIG1 may be a downstream target of RA signaling (2). RAIG1 is expressed in lung tissue and in several lung cancer cell lines, and encodes a seven-transmembrane glycoprotein presumed to be a lung tumor suppressor gene (3,4).

  1. Jörissen, H. et al. (2009) BMC Cancer 9, 200.
  2. Xu, J. et al. (2005) Am J Respir Cell Mol Biol 32, 381-7.
  3. Veling, G. et al. (1992) Kinderkrankenschwester 11, 118-20.
  4. Ye, X. et al. (2009) Cancer Biol Ther 8, 951-62.

Application References

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