Cell Signaling Technology

Product Pathways - Neuroscience

PINK1 (N4/15.11) Mouse mAb #5660

Applications Reactivity Sensitivity MW (kDa) Isotype
W IP H Transfected Only 60, 50 Mouse IgG1

Applications Key:  W=Western Blotting  IP=Immunoprecipitation
Reactivity Key:  H=Human
Species cross-reactivity is determined by western blot. Species enclosed in parentheses are predicted to react based on 100% sequence homology.

Protocols

Specificity / Sensitivity

PINK1 (N4/15.11) Mouse mAb recognizes transfected levels of total PINK1 protein. Endogenous levels of PINK1 have not been detected with this antibody so far.

Source / Purification

Monoclonal antibody is produced by immunizing animals with recombinant protein specific to the amino terminus of human PINK1 protein.

Western Blotting

Western Blotting

Western blot analysis of extracts from COS-7 cells mock transfected (-) or transfected with human PINK1 (+) using PINK1 (N4/15.11) Mouse mAb.

IF-IC

IF-IC

Confocal immunofluorescent analysis of 293 cells transfected with human PINK1 using PINK1 (N4/15.11) Mouse mAb (green). Actin filaments were labeled with DY-554 phalloidin (red). Blue pseudocolor = DRAQ5® #4084 (fluorescent DNA dye).

Background

PTEN induced putative kinase 1, PINK1, is a mitochondrial serine/threonine kinase involved in the normal function and integrity of mitochondria, as well as in reduction of cytochrome c release from mitochondria (1-3). PINK1 phosphorylates Parkin and promotes its translocation to mitochondria (2). Research studies have shown that mutations in PINK1 are linked to autosomal recessive early onset Parkinson’s disease, and are associated with loss of protective function, mitochondrial dysfunction, aggregation of α-synuclein, as well as proteasome dysfunction (1,3).

  1. Liu, W. et al. (2009) PLoS One 4, e4597.
  2. Kim, Y. et al. (2008) Biochem Biophys Res Commun 377, 975-80.
  3. Petit, A. et al. (2005) J Biol Chem 280, 34025-32.

Application References

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Companion Products

For Research Use Only. Not For Use In Diagnostic Procedures.

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