Cell Signaling Technology

Product Pathways - PI3K / Akt Signaling

PDK1 (D37A7) Rabbit mAb #5662

Applications Reactivity Sensitivity MW (kDa) Isotype
W H M R Hm Mk Endogenous 58-68 Rabbit IgG

Applications Key:  W=Western Blotting
Reactivity Key:  H=Human  M=Mouse  R=Rat  Hm=Hamster  Mk=Monkey
Species cross-reactivity is determined by western blot. Species enclosed in parentheses are predicted to react based on 100% sequence homology.

Protocols

Specificity / Sensitivity

PDK1 (D37A7) Rabbit mAb detects endogenous levels of total PDK1 protein.

Source / Purification

Monoclonal antibody is produced by immunizing animals with a synthetic peptide corresponding to residues near the carboxy terminus of human PDK1 protein.

Western Blotting

Western Blotting

Western blot analysis of extracts from various cell lines using PDK1 (D37A7) Rabbit mAb.

Western Blotting

Western Blotting

Western blot analysis of extracts from HCT 116 wild-type and HCT 116 PDK1-/- cells using PDK1 (D37A7) Rabbit mAb (upper) and Akt (pan) (C67E7) Rabbit mAb #4691 (lower). (HCT 116 wild-type and HCT 116 PDK1-/- cells were kindly provided by Dr. Bert Vogelstein, Johns Hopkins University, Baltimore, MD).

Background

Phosphoinositide-dependent protein kinase 1 (PDK1) plays a central role in many signal transduction pathways (1,2) including the activation of Akt and the PKC isoenzymes p70 S6 kinase and RSK (3). Through its effects on these kinases, PDK1 is involved in the regulation of a wide variety of processes, including cell proliferation, differentiation and apoptosis.

  1. Belham, C. et al. (1999) Curr. Biol. 9, R93-R96.
  2. Toker, A. and Newton, A.C. (2000) Cell 103, 185-188.
  3. Williams, M.R. et al. (2000) Curr. Biol. 10, 439-448.

Application References

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Companion Products

For Research Use Only. Not For Use In Diagnostic Procedures.

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