Cell Signaling Technology

Product Pathways - PI3K / Akt Signaling

NDRG2 Antibody #5667

Applications Reactivity Sensitivity MW (kDa) Source
W H M R Endogenous 45 Rabbit

Applications Key:  W=Western Blotting
Reactivity Key:  H=Human  M=Mouse  R=Rat
Species cross-reactivity is determined by western blot. Species enclosed in parentheses are predicted to react based on 100% sequence homology.

Protocols

Specificity / Sensitivity

NDRG2 Antibody recognizes endogenous levels of total NDRG2 protein.

Source / Purification

Polyclonal antibodies are produced by immunizing animals with a synthetic peptide corresponding to residues surrounding Lys25 of human NDRG2 protein. Antibodies are purified by protein A and peptide affinity chromatography.

Western Blotting

Western Blotting

Western blot analysis of extracts from 293T cells, mock transfected (-) or transfected with a mouse NDRG2 construct (+), using NDRG2 Antibody.

Western Blotting

Western Blotting

Western blot analysis of extracts from various cell lines using NDRG2 Antibody.

Background

The NDRG (N-Myc downstream-regulated gene) family, consisting of NDRG1, NDRG2, NDRG3, and NDRG4, are a group of structurally related proteins with roles in cell proliferation, differentiation, apoptosis, stress responses, and cell migration/metastasis (1-3). NDRG1 was originally identified as a protein that was up-regulated in N-Myc knockout mice (1). Proteins in the NDRG family, particularly NDRG1 and NDRG2, have been reported to be down-regulated in various cancer tissues, and have been suggested to function as a tumor suppressors (4,5).

Expression of NDRG1 and NDRG2 is up-regulated by p53 and HIF-1, and contributes to apoptosis driven by those pathways (6-11). NDRG1 and NDRG2 are also associated with pathological conditions in the nervous system. Nonsense mutation of the NDRG1 gene has been shown to cause hereditary motor and sensory neuropathy-Lom (HMSNL), which is supported by studies demonstrating the role of NDRG1 in maintaining myelin sheaths and axonal survival (12, 13). Elevated expression of NDRG2 has been observed with Alzheimer's disease (14). Both NDGR1 and NDGR2 are phosphorylated at multiple sites by Akt and/or SGK1, although the precise physiological role of this phosphorylation is not known (15, 16).

  1. Shimono, A. et al. (1999) Mech Dev 83, 39-52.
  2. Qu, X. et al. (2002) Mol Cell Biochem 229, 35-44.
  3. Zhou, R.H. et al. (2001) Genomics 73, 86-97.
  4. Yao, L. et al. (2008) Acta Biochim Biophys Sin (Shanghai) 40, 625-35.
  5. Ellen, T.P. et al. (2008) Carcinogenesis 29, 2-8.
  6. Kurdistani, S.K. et al. (1998) Cancer Res 58, 4439-44.
  7. Park, H. et al. (2000) Biochem Biophys Res Commun 276, 321-8.
  8. Stein, S. et al. (2004) J Biol Chem 279, 48930-40.
  9. Cangul, H. (2004) BMC Genet 5, 27.
  10. Wang, L. et al. (2008) Cell Physiol Biochem 21, 239-50.
  11. Liu, N. et al. (2008) Nucleic Acids Res 36, 5335-49.
  12. Kalaydjieva, L. et al. (2000) Am J Hum Genet 67, 47-58.
  13. Okuda, T. et al. (2004) Mol Cell Biol 24, 3949-56.
  14. Mitchelmore, C. et al. (2004) Neurobiol Dis 16, 48-58.
  15. Murray, J.T. et al. (2004) Biochem J 384, 477-88.
  16. Burchfield, J.G. et al. (2004) J Biol Chem 279, 18623-32.

Application References

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Companion Products

For Research Use Only. Not For Use In Diagnostic Procedures.

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