Cell Signaling Technology
XP Monoclonal Antibody

Product Pathways - Protein Folding

HOP (D10E2) XP® Rabbit mAb #5670

Applications Reactivity Sensitivity MW (kDa) Isotype
W IF-IC F H Mk B Endogenous 64 Rabbit IgG

Applications Key:  W=Western Blotting  IF-IC=Immunofluorescence (Immunocytochemistry)  F=Flow Cytometry
Reactivity Key:  H=Human  Mk=Monkey  B=Bovine
Species cross-reactivity is determined by western blot. Species enclosed in parentheses are predicted to react based on 100% sequence homology.

Protocols

* Product-specific protocol.

Specificity / Sensitivity

HOP (D10E2) XP® Rabbit mAb recognizes endogenous levels of total HOP protein. This antibody is not known to cross-react with other heat shock proteins.

Source / Purification

Monoclonal antibody is produced by immunizing animals with a synthetic peptide corresponding to residues surrounding Pro391 of human HOP protein.

Western Blotting

Western Blotting

Western blot analysis of whole cell extracts from various cell lines using HOP (D10E2) XP® Rabbit mAb.

Flow Cytometry

Flow Cytometry

Flow cytometric analysis of untreated HeLa cells using HOP (D10E2) XP® Rabbit mAb (blue) compared to Rabbit (DA1E) mAb IgG XP® Isotype Control #3900 (red).

IF-IC

IF-IC

Confocal immunofluorescent analysis of HeLa cells using HOP (D10E2) XP® Rabbit mAb (green). Actin filaments were labeled with DY-554 phalloidin (red). Blue pseudocolor = DRAQ5® #4084 (fluorescent DNA dye).


Background

HOP, also known as stress-induced phospho protein 1 (STIP), is a co-chaperone to the major heat shock proteins, Hsp70 and Hsp90, and appears in early receptor complexes (1,2). Through mutual binding to both Hsp70 and Hsp90, Hop functions as an adaptor that can integrate Hsp70 and Hsp90 interactions (3,4). HOP is an abundant and highly conserved protein which is composed of three tetratricopeptide repeat (TPR) domains (TPR1, TPR2a and TPR2b) and two DP repeat domains (DP1 and DP2), whose function has not been fully resolved (5).

  1. Smith, D.F. et al. (1993) Mol Cell Biol 13, 869-76.
  2. Smith, D.F. (1993) Mol Endocrinol 7, 1418-29.
  3. Chen, S. and Smith, D.F. (1998) J Biol Chem 273, 35194-200.
  4. Nelson, G.M. et al. (2003) Cell Stress Chaperones 8, 125-33.
  5. Carrigan, P.E. et al. (2005) J Biol Chem 280, 8906-11.

Application References

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Companion Products

For Research Use Only. Not For Use In Diagnostic Procedures.

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