Cell Signaling Technology

Product Pathways - Protein Stability

TIMP3 (D74B10) Rabbit mAb #5673

Applications Reactivity Sensitivity MW (kDa) Isotype
W H M R Mk Endogenous 20, 25 kDa Rabbit IgG

Applications Key:  W=Western Blotting
Reactivity Key:  H=Human  M=Mouse  R=Rat  Mk=Monkey
Species cross-reactivity is determined by western blot. Species enclosed in parentheses are predicted to react based on 100% sequence homology.

Protocols

Specificity / Sensitivity

TIMP3 (D74B10) Rabbit mAb detects endogenous levels of total TIMP3 protein.

Source / Purification

Monoclonal antibody is produced by immunizing animals with a synthetic peptide corresponding residues surrounding Lys53 of human TIMP3.

Western Blotting

Western Blotting

Western blot analysis of extracts from A-431, NIH/3T3 and COS-7 cells, using TIMP3 (D74B10) Rabbit mAb.

Background

TIMPs are members of the family of tissue inhibitor of matrix metalloproteinases (MMPs) that includes TIMP1, TIMP2, TIMP3, and TIMP4. The main function of TIMPs is their inhibitory effect on MMPs. TIMPs irreversibly inactivate MMPs by direct binding to their catalytic zinc cofactor and resultant inhibition of proteinase function (1,2). In addition to MMP inhibition, TIMPs have also been shown to interact with various membrane receptors on the cell surface. Some of these interactions include: TIMP1 with CD63, TIMP2 with α3β1 integrin, and TIMP3 with VEGFR2, all of which result in distinct cellular effects (3). TIMPs are involved in a wide variety of biological functions, such as tumor angiogenesis and progression (4,5), wound healing, and vascular remodeling (6,7). Mutations in TIMP3 are associated with Sorsby's fundus dystrophy (8,9).

  1. Nagase, H. et al. (2006) Cardiovasc Res 69, 562-73.
  2. Visse, R. and Nagase, H. (2003) Circ Res 92, 827-39.
  3. Stetler-Stevenson, W.G. (2008) Sci Signal 1, re6.
  4. Noel, A. et al. (2004) J Clin Pathol 57, 577-84.
  5. Hojilla, C.V. et al. (2003) Br J Cancer 89, 1817-21.
  6. Gill, S.E. and Parks, W.C. (2008) Int J Biochem Cell Biol 40, 1334-47.
  7. Raffetto, J.D. and Khalil, R.A. (2008) Biochem Pharmacol 75, 346-59.
  8. Weber, B.H. et al. (1994) Nat Genet 8, 352-6.
  9. Yeow, K.M. et al. (2002) Matrix Biol 21, 75-88.

Application References

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Companion Products

For Research Use Only. Not For Use In Diagnostic Procedures.

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