Cell Signaling Technology
XP Monoclonal Antibody

Product Pathways - Metabolism

Pdx1 (D59H3) XP® Rabbit mAb #5679

Applications Reactivity Sensitivity MW (kDa) Isotype
W IP IF-IC H R Endogenous 42 Rabbit IgG

Applications Key:  W=Western Blotting  IP=Immunoprecipitation  IF-IC=Immunofluorescence (Immunocytochemistry)
Reactivity Key:  H=Human  R=Rat
Species cross-reactivity is determined by western blot. Species enclosed in parentheses are predicted to react based on 100% sequence homology.

Protocols

Specificity / Sensitivity

Pdx1 (D59H3) XP® Rabbit mAb detects endogenous levels of total Pdx1 protein.

Source / Purification

Monoclonal antibody is produced by immunizing animals with a synthetic peptide corresponding to residues surrounding Val274 of human Pdx1 protein.

Western Blotting

Western Blotting

Western blot analysis of extracts from INS-1 cells using Pdx1 (D59H3) XP® Rabbit mAb.

IF-IC

IF-IC

Confocal immunofluorescent analysis of INS-1 cells (left) and KNRK cells (right) using Pdx1 (D59H3) XP® Rabbit mAb (green). Actin filaments were labeled with DY-554 phalloidin (red). Blue pseudocolor = DRAQ5® #4084 (fluorescent DNA dye).

Background

The pancreatic duodenal homeobox gene-1 (Pdx1) is a transcription factor that contributes to pancreas development, pancreatic β-cell differentiation, and mature β-cell function (1,2). It plays an essential role in the commitment of endoderm to a pancreatic and later β-cell phenotype (2,3). In the mature pancreas, Pdx1 expression is more restricted to the pancreatic β-cells (3), where it promotes the expression of genes important for β-cell functions such as insulin, glucokinase, and Glut2 (4-6). Mutations of the corresponding Pdx1 gene may be associated with diabetes and cases of pancreatic insufficiency (7).

  1. Kaneto, H. et al. (2008) Endocr J 55, 235-52.
  2. Habener, J.F. et al. (2005) Endocrinology 146, 1025-34.
  3. Guz, Y. et al. (1995) Development 121, 11-8.
  4. Ohlsson, H. et al. (1993) EMBO J 12, 4251-9.
  5. Watada, H. et al. (1996) Diabetes 45, 1826-31.
  6. Waeber, G. et al. (1996) Mol Endocrinol 10, 1327-34.
  7. Cockburn, B.N. et al. (2004) J Clin Endocrinol Metab 89, 971-8.

Application References

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Companion Products

For Research Use Only. Not For Use In Diagnostic Procedures.

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