Product Pathways - Translational Control
PERK (D11A8) Rabbit mAb #5683
|5683S||100 µl (10 western blots)||---||In Stock||---|
|5683P||40 µl (4 western blots)||---||In Stock||---|
|5683||carrier free and custom formulation / quantity||email request|
Already purchased this product? Write a Review.
Species cross-reactivity is determined by western blot.
Applications Key: W=Western Blotting, IP=Immunoprecipitation, IHC-P=Immunohistochemistry (Paraffin)
Specificity / Sensitivity
PERK (D11A8) Rabbit mAb recognizes endogenous levels of total PERK protein. Staining of red blood cells has been observed. The specificity of this staining is unknown.
Source / Purification
Monoclonal antibody is produced by immunizing animals with a synthetic peptide corresponding to residues surrounding Leu156 of human PERK protein.
Western blot analysis of extracts from various cell lines using PERK (D11A8) Rabbit mAb.
Protein kinase-like endoplasmic reticulum kinase (PERK) is an eIF2α kinase and transmembrane protein resident in the endoplasmic reticulum (ER) membrane that couples ER stress signals to translation inhibition (1-3). ER stress increases the activity of PERK, which then phosphorylates eIF2α to promote reduced translation. Research studies have demonstrated that PERK-deficient mice have defects in pancreatic β cells several weeks after birth, suggesting a role for PERK-mediated translational control in protecting secretory cells from ER stress (4). PERK activation during ER stress correlates with autophosphorylation of its cytoplasmic kinase domain (1-3). Phosphorylation of PERK at Thr980 serves as a marker for its activation status.
Have you published research involving the use of our products? If so we'd love to hear about it. Please let us know!
- 3192 PERK (C33E10) Rabbit mAb
- 3179 Phospho-PERK (Thr980) (16F8) Rabbit mAb
- 3597 Phospho-eIF2α (Ser51) (119A11) Rabbit mAb
- 9721 Phospho-eIF2α (Ser51) Antibody
- 5554 CHOP (D46F1) Rabbit mAb
- 2895 CHOP (L63F7) Mouse mAb
- 3177 BiP (C50B12) Rabbit mAb
- 3183 BiP Antibody
- 2679 Calnexin (C5C9) Rabbit mAb
- 2433 Calnexin Antibody
- 2891 Calreticulin Antibody
- 3264 Ero1-Lα Antibody
- 2886 ERp44 Antibody
- 3294 IRE1α (14C10) Rabbit mAb
- 2157 MBTPS2 Antibody
- 2446 PDI Antibody
- 9997 Tris Buffered Saline with Tween® 20 (TBST-10X)
- 9998 BSA
- 9999 Nonfat Dry Milk
- 7071 Phototope®-HRP Western Blot Detection System, Anti-rabbit IgG, HRP-linked Antibody
- 7074 Anti-rabbit IgG, HRP-linked Antibody
- 7720 Prestained Protein Marker, Broad Range (Premixed Format)
- 7727 Biotinylated Protein Ladder Detection Pack
- 7003 20X LumiGLO® Reagent and 20X Peroxide
- 8112 SignalStain® Antibody Diluent
- 8114 SignalStain® Boost IHC Detection Reagent (HRP, Rabbit)
- 5425 Normal Goat Serum
For Research Use Only. Not For Use In Diagnostic Procedures.
Cell Signaling Technology® is a trademark of Cell Signaling Technology, Inc.