Cell Signaling Technology

Product Pathways - Translational Control

PERK (D11A8) Rabbit mAb #5683

Applications Reactivity Sensitivity MW (kDa) Isotype
W IP IHC-P H Endogenous 140 Rabbit IgG

Applications Key:  W=Western Blotting  IP=Immunoprecipitation  IHC-P=Immunohistochemistry (Paraffin)
Reactivity Key:  H=Human
Species cross-reactivity is determined by western blot. Species enclosed in parentheses are predicted to react based on 100% sequence homology.

Protocols

Specificity / Sensitivity

PERK (D11A8) Rabbit mAb recognizes endogenous levels of total PERK protein. Staining of red blood cells has been observed. The specificity of this staining is unknown.

Source / Purification

Monoclonal antibody is produced by immunizing animals with a synthetic peptide corresponding to residues surrounding Leu156 of human PERK protein.

Western Blotting

Western Blotting

Western blot analysis of extracts from various cell lines using PERK (D11A8) Rabbit mAb.

IHC-P (paraffin)

IHC-P (paraffin)

Immunohistochemical analysis of paraffin-embedded human breast carcinoma using PERK (D11A8) Rabbit mAb.

Background

Protein kinase-like endoplasmic reticulum kinase (PERK) is an eIF2α kinase and transmembrane protein resident in the endoplasmic reticulum (ER) membrane that couples ER stress signals to translation inhibition (1-3). ER stress increases the activity of PERK, which then phosphorylates eIF2α to promote reduced translation. Research studies have demonstrated that PERK-deficient mice have defects in pancreatic β cells several weeks after birth, suggesting a role for PERK-mediated translational control in protecting secretory cells from ER stress (4). PERK activation during ER stress correlates with autophosphorylation of its cytoplasmic kinase domain (1-3). Phosphorylation of PERK at Thr980 serves as a marker for its activation status.

  1. Harding, H. et al. (1999) Nature 397, 271-274.
  2. Shi, Y. et al. (1998) Mol. Cell. Biol. 18, 7499-7509.
  3. Harding, H. et al. (2000) Mol. Cell 5, 897-904.
  4. Harding, H. et al. (2001) Mol. Cell 7, 1153-1163.

Application References

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Companion Products

For Research Use Only. Not For Use In Diagnostic Procedures.

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