Cell Signaling Technology

Product Pathways - Phosphatases

PPP2R4 (5G3) Mouse mAb #5686

Applications Reactivity Sensitivity MW (kDa) Isotype
W H M Mk Endogenous 40 Mouse IgG2a

Applications Key:  W=Western Blotting
Reactivity Key:  H=Human  M=Mouse  Mk=Monkey
Species cross-reactivity is determined by western blot. Species enclosed in parentheses are predicted to react based on 100% sequence homology.

Protocols

Specificity / Sensitivity

PPP2R4 (5G3) Mouse mAb recognizes endogenous levels of total PPP2R4 protein. This antibody does not cross-react with other PP2A proteins.

Source / Purification

Monoclonal antibody is produced by immunizing animals with recombinant protein specific to the mouse PPP2R4 protein.

Western Blotting

Western Blotting

Western blot analysis of extracts from MCF7, Hep G2, and HT-29 cells using PPP2R4 (5G3) Mouse mAb.

Background

Serine/threonine protein phosphatase 2A regulatory subunit 4 (PPP2R4) is one of the constituents of the regulatory subunit B' of PP2A (1). PPP2R4 is also known as the phosphatase activator (PTPA) and known to have the peptidyl prolyl isomerase (PPIase) activity (2-3). PPIases catalyze cis-trans isomerization of peptide bonds of proline residues in the polypeptide chain; this leads to the rapid folding of proteins (2). The ATPase activity of PPP2R4 is required for the phosphotyrosyl phosphatase activity of PP2A (2-4). Evidence suggests that PPP2R4 induces apoptosis independent of the canonical PP2A-mediated pathways (4).

  1. Jessus, C. et al. (1989) Biochem J 260, 45-51.
  2. Cayla, X. et al. (1990) Biochemistry 29, 658-67.
  3. Chao, Y. et al. (2006) Mol Cell 23, 535-46.
  4. Azam, S. et al. (2007) Apoptosis 12, 1243-55.

Application References

Have you published research involving the use of our products? If so we'd love to hear about it. Please let us know!

Companion Products

For Research Use Only. Not For Use In Diagnostic Procedures.

Products