Product Pathways - Phosphatases
PPP2R5D (H5D12) Mouse mAb #5687
PhosphoSitePlus® protein, site, and accession data: PPP2R5D
| Applications | Reactivity | Sensitivity | MW (kDa) | Isotype |
|---|---|---|---|---|
| W IP | H M R Mk | Endogenous | 66, 69 kDa | Mouse IgG1 |
Applications Key:
W=Western Blotting
IP=Immunoprecipitation
Reactivity Key:
H=Human
M=Mouse
R=Rat
Mk=Monkey
Species cross-reactivity is determined by western blot. Species enclosed in parentheses are predicted to react based on 100% sequence homology.
Protocols
Specificity / Sensitivity
PPP2R5D (H5D12) Rabbit mAb recognizes endogenous levels of total PPP2R5D/PP2A, B'-δ/B56-δ protein. This antibody cross-reacts with PPP2R4 and PPP2R2A protein.
Source / Purification
Monoclonal antibody is produced by immunizing animals with recombinant protein specific to the amino terminus of human PPP2R5D protein.
Western Blotting
Western blot analysis of extracts from MCF7, HeLa, and Hep G2 cells using PPP2R5D (H5D12) Mouse mAb.
Background
Protein Phosphatase 2A subunit isoform R5-δ (PPP2R5D) belongs to the R5/B'/B56 family of the regulatory subunit B of serine/threonine phosphatase 2A (1). PPP2R5D (B-δ) is structurally related to the other components of subunit B, B-α. PPP2R5D lacks the WD-40 repeats that are important for protein-protein interaction (1-2). Structural differences may contribute to distinct down stream effects (3) and directing PP2A nuclear localization (4). Current evidence suggests that in contrast to B-α, PPP2R5D modulates the TGF-β/Actividin/Nodal signaling pathways by restricting the downstream activities of the type I TGF-β receptors, ALK4, and ALK5 (3)
- McCright, B. and Virshup, D.M. (1995) J Biol Chem 270, 26123-8.
- Neer, E.J. et al. (1994) Nature 371, 297-300.
- Batut, J. et al. (2008) Development 135, 2927-37.
- McCright, B. et al. (1996) J Biol Chem 271, 22081-9.
Application References
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Companion Products
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- 1067 PP2A C Subunit Blocking Peptide
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- 5689 PPP2R2A (2G9) Mouse mAb
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- 7074 Anti-rabbit IgG, HRP-linked Antibody
- 7720 Prestained Protein Marker, Broad Range (Premixed Format)
- 7727 Biotinylated Protein Ladder Detection Pack
- 7003 20X LumiGLO® Reagent and 20X Peroxide
For Research Use Only. Not For Use In Diagnostic Procedures.
